Dissemination of Integron Class 1 among XDR isolates of P. aeruginosa in Najaf City

Abstract

Background: Integrons are gene capture systems observed in chromosomes, plasmids, and transposons, where identified and capture multiple gene cassettes. Integerons are consists of 3 elements: the initial is integrase gene (int1) encoding integrase enzyme (int1), which is essential to insertion or deletion of specific site. The second element is attI as integron-connected recombination site. The 3rd element is promoter (Pc), which is required in the expression of cassette-connected genes.                                                                                                             Aim of the Study: This thesis designed to examine the occurrence of integron class one genes among XDR isolates of P. aeruginosa.                Methods: An overall of 79 specimens were isolated from patients during the period of study and the specimens were carried to the laboratory. By monoplex PCR assay, the 19 XDR isolates of P. aeruginosa were investigated for the presence of integrons class one using intI1primer.                                                                  Results: This result found that 8 isolates are isolated from burns, 7 isolates are from urine, 4 isolates are from wounds. The PCR results show that 17 (89.5%) of the isolates harbored integrase (intI1) gene, while two isolate (10.5%) was negative for intI1 gene.                            Conclusions: Present result showed elevated occurrence of integrons class one among XDR isolates of P.aeruginosa.