In vitro Studies on Antioxidant Potential of Aqueous Extract of Bridelia ferruginea Stem Bark in Brain and Liver of Wistar-albino Rats using Sodium Nitroprusside as Pro-oxidant

Abstract

Thiobarbituric acid reactive species (TBARS) assay was used to study the antioxidant potential of Bridelia ferruginea, a widely used medicinal plant in Nigeria, sub-tropical Africa and parts of Asia. The aqueous extract of Bridelia ferruginea stem bark showed inhibition against the formation of TBARS induced by the pro-oxidant, sodium nitroprusside, in the liver and brain tissue homogenates of the locally bred male and female albino-Wistar rats. The inhibition of TBARS is an indication of the antioxidant potential of the plant extract. The extent of antioxidant potential depends on concentrations, showing varying degrees of inhibition with different concentrations. It showed a 54.16% inhibition in the liver and 60.65% in the brain, both at a concentration of 0.33 mg/ml, with IC50 values of 3.00 ± 1.58 mg/ml and 2.99 ± 1.59 mg/ml for the liver and brain homogenates respectively. The results suggest the effectiveness of the aqueous extract of Bridelia ferruginea stem bark in reversing the effect of lipid peroxidation that may result from sodium nitroprusside overload.