Optimized in vitro Micropropagation Protocol for Acorus calamus L. Using Rhizome Explants

Abstract

The present investigation has been conducted on “in-vitro micro propagation of Acoruscalamus L. using rhizome explants”. This study was undertaken to identify asuitable media combinations for the micro propagation of Acorus calamus L. The authenticated mother plant of Acorus calamus L. was collected from the Dhanvantari Nursery, Kedgaon. Tal - Nagar, Dist.- Ahilyanagar (Maharashtra). The surface sterilization treatments of 0.1% Bavistin along with 0.1% HgCl2 and 70% ethanol were found effective for Acorus calamus L. The MS media supplemented with different concentrations of BAP was utilized for initiation. MS medium supplemented with 2.0mg/L, 2.5mg/L and 3.0mg/L BAP shows the maximum direct regeneration. Then the well grown cultures were transferred to the new medium with different concentration of BAP and NAA. The Multiplication of shoots using 3.0 mg/L BAP + 3.0 mg/L NAA provided the highest multiplication rate, with increased number of shoots per explant. Following successful multiplication, the shoots were moved to MS media with varying IBA concentrations (1.0 mg/L and 1.5 mg/L). Both treatments showed significant rooting, with 1.5 mg/L IBA showing the greatest root length and density. These conditions collectively support efficient clonal propagation and hold promise for scalable production and germplasm conservation of Acorus calamus L. Future work could focus on improving acclimatization and field performance.