Production of Modified Rapid Serum Agglutination Antigen for Sero-diagnosis of Contagious Bovine Pleuropneumonia (CBPP)

Abstract

Aims: This work was conducted to develop rapid serum agglutination test for Contagious Bovine Pleuropneumonia (CBPP) serodiagnosis. Study Design: Production of two types of antigen, one from T1/44 reference strain of Mycoplasma mycoides sub sp.  mycoides (Mmm) and the other from local strain (RH).  Place and Duration of Study: This study was carried out at Mycoplasma department Central Veterinary Research Lab (CVRL) in Sudan during 2016-2018. Methodology: The antigens have been developed using standard methods and evaluated using standard reference serum, c.ELISA and Latex Agglutination test. Statistical analysis using Chi-square test and Kappa agreement were applied.   Results: The sensitivity of the developed stained antigen from T1/44 strain (SAT1) was 100% in comparison of 92% and 95% of SAT2 (RH local strain) and LAT, respectively, The developed SAT2 antigen revealed satisfied specificity results (70%), which is higher than SAT1 (31%) and LAT (59.6%). Conclusion: From these results we found that SAT2 which is produced from local strain- proved to be more specific and highly sensitive. The SAT2 combines low cost and high specificity and easy applicable in field, without need for any specialist training or equipment.