Response Surface Methodology Based Optimization of a New Isolate Bacillus pumilus ZR LS S2 with Fibrinolytic Activity

Abstract

Aims: The work aims to isolate potential fibrinolytic enzyme producing isolates from various samples with the aim of developing a suitable optimization strategy using response surface methodology. Study Design:  Plackett- Burman and Face centered central composite design was used. Place and Duration of Study: Department of Life Sciences, University of Calicut. Methodology: Various samples were screened using fibrin agar plates for the isolation of fibrinolytic enzyme producing bacteria and was identified by 16 s rRNA sequencing. Further the physical parameters and media components were optimized using Plackett- Burman design and face centered central composite design. Results: A novel isolate with fibrinolytic potential was isolated and was identified as Bacillus pumilus ZR LS S2. A novel media was formulated using this data, for the production of fibrinolytic enzyme, which contains peptone, casein, MgSO4 and NaCl. The isolate produced 6738.384 U/ml of enzyme in optimized conditions.  Conclusion: Initial studies were performed for the production of the fibrinolytic enzyme by the novel isolate, further studies are required to effectively validate the potential of the fibrinolytic enzyme and to develop effective application for the same.