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Research Article Open access CC BY 4.0

Isolation and Identification of Cellulytic Fungi from Agrowastes and Sawmill Soils

Ekundayo Temitope Cyrus, Arotupin Daniel Juwon

Biotechnology Journal International · pp. 147–159 · Published 6 May 2015

10.9734/BBJ/2015/17575

Abstract

Five agricultural wastes and sawmill soil were investigated for fungi capable of producing cellulase. Isolation was carried using serial dilution techniques and isolates were screened for cellulase production on carboxymethyl cellulose-containing agar plates. Copious isolates were further subjected to submerged cultivation for quantitative evaluation of cellulase biosynthesis. Highest fungal load was 6.67±0.33×106 sfu/g (Sawmill soil) and the least was 2.33±0.33×106 sfu/g (yam peels). A total of sixty seven fungi were identified from the samples. The isolates were of fifteen fungal species namely Mucor mucedo, Aspergillus niger, A. repens, A. flavus, A. parasiticus, Articulospora inflata, Gonatobotrys simplex, Gyrothrix circinata, Dendrospora erecta, Penicillium notatum, P. italicum, Varicosporium elodea, Gonatobotryum apicolatum, Mucor racemosus and Rhizopus nigricans. Aspergillus had the highest occurrence (36.11%), while the least occurrence was Varicosporium and Gonatobotryum (2.78%). Highest cellulase activity ratio was exhibited by Aspergillus niger (1.90) and the least by Aspergillus repens (1.04) on plate screening. The highest cellulase producer among selected isolates under submerged growth was Gonatobotrys simplex (1.2143±0.02 U/ml), followed by Aspergillus niger (1.1429±0.01 U/ml) and the least by Aspergillus parasiticus (0.8265±0.01 U/ml). Submerged protein content was highest in Aspergillus niger culture and least in Penicillium notatum. The study has revealed array of cellulytic fungi from agricultural wastes and sawmill soil.

Cellulytic fungi agricultural wastes cellulose sawmill soil submerged cultivation

References (24)

  1. 1 PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT [DOI]
  2. 2 Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar [DOI]
  3. 3 Microbial Cellulose Utilization: Fundamentals and Biotechnology [DOI]
  4. 4 Toward an aggregated understanding of enzymatic hydrolysis of cellulose: Noncomplexed cellulase systems [DOI]
  5. 5 Lignocellulosic residues: Biodegradation and bioconversion by fungi [DOI]
  6. 6 Microbial Cellulases and Their Industrial Applications [DOI]
  7. 7 Lignocellulose biotechnology: issues of bioconversion and enzyme production [DOI]
  8. 8 Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viride under SSF and its detergent compatibility [DOI]
  9. 9 Nutrient effects on production of cellulolytic enzymes by Aspergillus niger [DOI]
  10. 10 SCREENING AND CHARACTERIZATION OF FUNGAL CELLULASES ISOLATED FROM THE NATIVE ENVIRONMENTAL SOURCE
  11. 11 Pilot-scale production of cellulase using Trichoderma reesei Rut C-30 in Fed-Batch mode
  12. 12 Optimization for cellulase production by Aspergillus niger using saw dust as substrate [DOI]
  13. 13 Optimization of the medium for the production of cellulase by the Trichoderma viride using submerged fermentation
  14. 14 Production and Partial Purification of Cellulase by Aspergillus niger and A. fumigatus Fermented in Coir waste and Sawdust [DOI]
  15. 15 ASSOCIATION OF FUNGI, BACTERIA AND ACTINOMYCETES WITH DIFFERENT COMPOSTS
  16. 16 Corn stover-enhanced cellulase production by Aspergillus niger NRRL 567 [DOI]
  17. 17 Production of cellulase from a thermophilic Bacillus sp. isolated from cow dung.
  18. 18 OPTIMIZATION OF PROCESS PARAMETERS FOR THE BIOSYNTHESIS OF CELLULASES BY TRICHODERMA VIRIDE
  19. 19 Evaluation of Microorganisms from Cassava Waste Water for Production of Amylase and Cellulase [DOI]
  20. 20 Optimization studies on mannanase production by Trichosporonoides oedocephalis in submerged state fermentation
  21. 21 Exploration of indigenous agrowastes for cellulase production by Aspergillus niger [DOI]
  22. 22 Isolation of Ascomycetous Fungi from a Tertiary Institution Campus Soil [DOI]
  23. 23 Investigation of the cellulases production by Aspergillus niger NSPR002 in different cultivation conditions.
  24. 24 Radiation mutagenesis and purification of xylanase produced by soil fungi [DOI]

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