Influence of Various Nitrogen Sources on Biomass and Lipid Production by Chlorella vulgaris
Biotechnology Journal International · pp. 1–13 · Published 10 Aug 2016
10.9734/BBJ/2016/21727Abstract
Chlorella vulgaris is a unicellular, photosynthetic fresh water green alga with high concentration of chlorophyll. This microalga synthesizes biomass by trapping energy from the sun. It has valuable components particularly pigments and protein thus, can be utilized in the nutraceutical, pharmaceutical and agricultural industry. The organism was obtained by blooming in a 10:90 mixture of cow dung extract and pond water from fresh water pond at the African Regional Aquaculture Centre [ARAC] at Aluu, Rivers State, Nigeria. Blooming was enhanced by intermittent manual aeration under natural illumination with a bank of fluorescent tubes emiting ca15 µE/m²/s each. The isolate was cultured using a synthetic medium and identified as Chlorella vulgaris on the basis of its molecular characteristics by Polymerase Chain Reaction [PCR] technique. The potential of producing biomass and lipid from Chlorella vulgaris using three different nitrogen sources namely potassium nitrate, urea and sodium nitrate in a synthetic medium were investigated. The best growth of about 279 mgL-1 cell dry matter and 5.27% lipid content was obtained with urea as compared to the other nitrogen sources. Potassium nitrate gave 68 mgL-1 cell dry matter with about 1.53% lipid content, while sodium nitrate resulted in 236 mgL-1 cell dry matter and 0.73% lipid content. The maximum specific growth rate (µ=0.198) with a doubling time of 5.05 was recorded with urea at a concentration of 0.055 mgL-1, NaNO3 at the same concentration with urea had (µ=0.182) and a doubling time of 5.5, but KNO3 showed the least specific growth rate (µ= 0.169) with doubling time of 6.33. Overall, urea gave higher yields of biomass and lipid, caused small fluctuations with the medium during the algal growth.
References (42)
- 1 Biodiesel from microalgae [DOI]
- 2 Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low‐cost photobioreactor [DOI]
- 3 Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production [DOI]
- 4 Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions [DOI]
- 5 Increase in Chlorella strains calorific values when grown in low nitrogen medium [DOI]
- 6 Effect of iron on growth and lipid accumulation in Chlorella vulgaris [DOI]
- 7 Effect of salt concentration on intracellular accumulation of lipids and triacylglyceride in marine microalgae Dunaliella cells [DOI]
- 8 High yield bio-oil production from fast pyrolysis by metabolic controlling of Chlorella protothecoides [DOI]
- 9 Microalgae — biotechnology and microbiology [DOI]
- 10 Biodiversity and application of microalgae [DOI]
- 11 Biomass production, total protein, chlorophylls, lipids and fatty acids of freshwater green and blue-green algae under different nitrogen regimes [DOI]
- 12 Cultivation of microalgae for oil production with a cultivation strategy of urea limitation [DOI]
- 13 Microalgae as food and supplement [DOI]
- 14 BIOCHEMICAL TAXONOMY AND MOLECULAR PHYLOGENY OF THE GENUS CHLORELLA SENSU LATO (CHLOROPHYTA) [DOI]
- 15 Heterotrophic production of biomass and lutein by Chlorella protothecoides on various nitrogen sources [DOI]
- 16 Oil Accumulation via Heterotrophic/Mixotrophic Chlorella protothecoides [DOI]
- 17 Lipid production by Chlorella vulgaris after a shift from nutrient-rich to nitrogen starvation conditions [DOI]
- 18 Phylogenetic relationship of Chlorella and Parachlorella gen. nov. (Chlorophyta, Trebouxiophyceae) [DOI]
- 19 Effects of temperature and nutrient regimes on biomass and lipid production by six oleaginous microalgae in batch culture employing a two-phase cultivation strategy [DOI]
- 20 An investigation of effect of replacing nitrate by urea in the growth and production of chlorophyll by Spirulina platensis [DOI]
- 21 Heterotrophic Culture of Chlorella protothecoides in Various Nitrogen Sources for Lipid Production [DOI]
- 22 High-cell-density cultivation of Schizochytrium sp. in an ammonium/pH-auxostat fed-batch system [DOI]
- 23 COMMERCIAL APPLICATION OF MICROALGAE
- 24 Ammonium and urea removal by Spirulina platensis [DOI]
- 25 Astaxanthin Production by Haematococcus pluvialis under Various Light Intensities and Wavelengths
- 26 Nitrogen Removal from Wastewaters by Microalgae Without Consuming Organic Carbon Sources
- 27 Scenedesmus dimorphus and Scenedesmus quadricauda : two potent indigenous microalgae strains for biomass production and CO2 mitigation - A study on their growth behavior and lipid productivity under different concentration of urea as nitrogen source.
- 28 A laboratory-scale cultivation of Chlorella and Spirulina using waste effluent from a fertilizer company in Nigeria [DOI]
- 29 Services of Algae to the Environment
- 30 Identification of the green alga, Chlorella vulgaris (SDC1) using cyanobacteria derived 16S rDNA primers: targeting the chloroplast [DOI]
- 31 Comparison Of Different Media Formulations On Growth, Morphology And Chlorophyll Content Of Green Alga, Chlorella Vulgaris
- 32 Effect of some nitrogen sources on growth and lipid of microalgae Chlorella sp. for biodiesel production.
- 33 Growth, survival and reproduction inChlorella vulgaris andC. variegata with respect to culture age and under different chemical factors [DOI]
- 34 Introduction of sorghum (Sorghum bicolor (L.) Moench) into China. [DOI]
- 35 Biosorption Model for Binary Adsorption Sites
- 36 Utilization of Different Nitrogen Sources by Cultures of Scenedesmus acuminatus
- 37 Kinetics of Removing Nitrogenous and Phosphorus Compounds from Swine Waste by Growth of Microalga, Spirulina platensis
- 38 Effects of nutrient levels on cell growth and secondary carotenoids formation in the freshwater green alga, Chlorococcum sp.
- 39 Influence of different Carbon and Nitrogen sources on growth and CO2fixation of microalgae
- 40 Elevation of the efficiency of cyanobacterial carbon dioxide removal by monoethanolamine solution
- 41 Effect of ionic copper toxicity on the growth of Green Alga, Selenastrum capricornutum
- 42 Microalgae: biotechnology and microbiology [DOI]
Cited by 12
Kulvinder Bajwa, Narsi R. Bishnoi, Anita Kirrollia · European Journal of Sustainable Development Research · 2018
Obioma Kenechukwu Agwa, Chimezie Jason Ogugbue, Enechojo Eunice Williams · International Journal of Agricultural Research · 2017
Muhamad Maulana Azimatun Nur, W. Muizelaar, P. Boelen · Journal of Applied Phycology · 2018
Osama M. Darwesh, Ibrahim A. Matter, Mohamed F. Eida · Applied Sciences · 2019
Department of Biology, Air Force Institute of Technology Kaduna Nigeriaж Department of Biochemistry, Ahmadu Bello University, Zaria, Kaduna State, Nigeria, D. D. Gaiya · Biotechnologia Acta · 2021
Obioma Kenechukwu Agwa, Chimezie Jason Ogugbue, Enechojo Eunice Williams · American Journal of Plant Sciences · 2018
Showing 6 of 12 known citations — external sources report more than can currently be individually listed.
Related research
- Evaluating Serum Urea, Creatinine and Hemoglobin Level in Chronic Renal Failure of Pre and Post Dialysis at St. Paul’s Hospital Millennium Medical College, Addis Ababa, Ethiopia — shares topic coverage
- Effects of Urea, Molasses and Fibrolytic Enzymes on Nutritional Value of Date Palm (Phoenix dactylifera) Leaves Silage — shares topic coverage
- Effect of Feeding Chemically and Biologically Treated Barley Straw on Some Hematological and Serum Biochemical Parameters of Karadi Lambs — shares topic coverage
- Formulation of Cost-effective Medium Using Urea as a Nitrogen Source for Arthrospira platensis Cultivation under Real Environment — shares topic coverage
- Supramolecular Catalysis with Chiral Mono- and Bis-(Thio)Urea-Derivatives — shares topic coverage
Article metrics
Real usage data collected on this platform.
55
Page views
0
PDF downloads
17
Outbound clicks
12
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.