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Research Article Open access CC BY 4.0

Influence of Solvents and Solvent Mixtures on the Content of Polyphenols and Antioxidant Activity of Capsella bursa-pastoris (L.) Medik. Extracts

Emir Horozić, Edina Huseinović, Lejla Mekić, Lamija Kolarević, Azra Hadžigrahić, Enida Karić, Merima Ibišević

Journal of Scientific Research and Reports · pp. 35–41 · Published 29 Aug 2023

10.9734/jsrr/2023/v29i91782

Abstract

Capsella bursa-pastoris (L.) Medik. (known as shepherd's purse) is a plant whose parts are used as medicine in herbal medicine. It is applicable as a medicine in the treatment of all forms of internal bleeding, for the treatment of hemorrhoids, excessive menstruation, but also for the usual stopping of nosebleeds. Through this research, the influence of organic solvents and their aqueous mixtures on the efficiency of polyphenol extraction and antioxidant activity was compared. The inhibition of free radicals was tested by the DPPH method, while the FRAP method was used to test the reduction potential. Analyzes have shown that water is the most effective solvent in the isolation of polyphenols from the aerial parts of shepherd's purse. Mixtures of organic solvents with water also showed high efficiency in the extraction of bioactive components, while the weakest results were obtained for extracts prepared in pure organic solvents.

Shepherd's purse TPC FRAP DPPH inhibition

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