Influence of Explants Type, Nodal Positions and Two Cytokinins on Cassava (Manihot esculenta, Crantz)  In vitro Organogenesis

Gilles Habib Todjro Cacaï; Jerome Anani Houngue; Serge S. Houédjissin; Fleur Gbèmandé Pativoh; Corneille Ahanhanzo

doi:10.9734/jabb/2023/v26i12669

Research Article Open access CC BY 4.0

Influence of Explants Type, Nodal Positions and Two Cytokinins on Cassava (Manihot esculenta, Crantz) In vitro Organogenesis

Gilles Habib Todjro Cacaï, Jerome Anani Houngue, Serge S. Houédjissin, Fleur Gbèmandé Pativoh, Corneille Ahanhanzo

Journal of Advances in Biology & Biotechnology · pp. 13–23 · Published 30 Dec 2023

10.9734/jabb/2023/v26i12669

Abstract

Cassava In vitro regeneration is essentially influenced by exogen factors as well as explants nature. This work aimed to evaluate explants type and positions influence, phytohormone on cassava plantlets regeneration in modified MS media. The apex and nodes of the position 3, 5, and 7 were initiated on Murashige and Skoog (MS) based media supplemented with Gamborg B5 vitamins and growth regulators such as naphthalene acetic acid (NAA), 6-benzylaminopurine (BAP) and zeatin (ZEAT) in different concentrations. The results indicated a significant difference (p=0.0001) among explants sprouted regarding the nodes position and the cultivar genotype during the time of culture. The highest rate of regenerated plantlets was found with nodes of position 5 (95.33%) followed respectively by nodes of the position 7 (93.33%), nodes of the position 3 (89.33%), and the apex (76%). Regarding the growth regulators, NAA (0.5 mg/l) + BAP (0.5 mg/l) combination was favorable for height growth (26.2cm), and leaves development (7.84 cm) in most of cultivars, whereas NAA (0.5 mg/l) + ZEAT (0.5 mg/l) combination was more favorable to rhizogenesis in overall cultivars. Among the cultivars, the best performance in stem height (26.2 cm), in leaf length (7.84 cm), and roots number (4.64) were observed with INA 1er cultivar. The cultivars RB89509, BEN 86052, and DOKU were less grown on MS supplemented with 0.5 mg/l of BAP or ZEAT. These findings could contribute of using nodes of the positions 5, 7 as potential explants and Zeatin for cassava plantlets rhizogenesis.

Cassava micropropagation In vitro culture nature of explant growth regulators plantlets regeneration

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Gilles Habib Todjro Cacaï, Jerome Anani Houngue, Serge S. Houédjissin, Fleur Gbèmandé Pativoh, Corneille Ahanhanzo (2023). Influence of Explants Type, Nodal Positions and Two Cytokinins on Cassava (Manihot esculenta, Crantz) In vitro Organogenesis. Journal of Advances in Biology & Biotechnology , 13–23. https://doi.org/10.9734/jabb/2023/v26i12669

Affiliations

Gilles Habib Todjro Cacaï — Department of Genetics and Biotechnology, Faculty of Science and Technique, Laboratory of Plant Biotechnology and Plant Breeding, University of Abomey-Calavi, Benin.
Jerome Anani Houngue — Department of Genetics and Biotechnology, Faculty of Science and Technique, Laboratory of Plant Biotechnology and Plant Breeding, University of Abomey-Calavi, Benin.
Serge S. Houédjissin — Department of Genetics and Biotechnology, Faculty of Science and Technique, Laboratory of Plant Biotechnology and Plant Breeding, University of Abomey-Calavi, Benin.
Fleur Gbèmandé Pativoh — Department of Genetics and Biotechnology, Faculty of Science and Technique, Laboratory of Plant Biotechnology and Plant Breeding, University of Abomey-Calavi, Benin.
Corneille Ahanhanzo — Department of Genetics and Biotechnology, Faculty of Science and Technique, Laboratory of Plant Biotechnology and Plant Breeding, University of Abomey-Calavi, Benin and Benin Center of Scientific Research and Innovation, Benin.

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