Skip to content
Research Article Open access CC BY 4.0

Callus Cell Proliferation and Explants Regeneration Using Broccoli Shoot Tip in vitro Culture: Biochemical and Antioxidant Properties

A. B. M. Sharif Hossain, Imdadul Haq, Mohammed Saad Aleissa, Nasir Adam Ibrahim, Kamaludin Bin Rashid

Current Journal of Applied Science and Technology · pp. 1–8 · Published 15 Dec 2015

10.9734/BJAST/2016/22665

Abstract

Significance of the Study: Nowadays cell or tissue culture has been successfully performed using different types of species like fruit, vegetable, ornamental and forest plant. Millions of explants can be produced by tissue or cell culture per year in any plant production industry. Combination of 6-Benzylaminopurine (BAP) and other plant growth regulators like 1-Naphthaleneacetic acid (NAA) or Indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) was used in the most of the research in tissue culture. Aims: The study was carried out to investigate the root, callus, shoot and leaf proliferation from the shoot tip in vitro culture using different IBA and BAP concentration. Methodology: Shoot tip slice was used to culture in the MS (Murashige and Skoog) media. Different IBA and BAP (0, 0.25, 0.50, 1.0, 1.50, 2.0 2.5, 3.0 and 3.5 mg/l) concentrations in combination with MS media were used. Results: The highest number (7.4) of shoot proliferation was observed in the concentration of IBA 0.25+BAP 2.5 mg/l combination. The maximum root proliferation was found in the concentration of IBA 0.25+ BAP 1.5. However, callus formation was observed better at the concentration of BAP 1-3.5 and IBA 1-3.5 mg/l combination than other combination of concentrations. The 2,2-diphenyl-2-picrylhydarzyl (DPPH) free radical scavenging potential was higher (70.1%) in leaves extract than in callus extracts (46.3%) at the concentration of 10 mg/ml. Besides, both extracts had lower DPPH free radical scavenging activity compared to the positive control, vitamin C and BHT. Conclusion: The present results conclude that it is better to use the combination of the IBA and BAP concentration to produce root, shoot, leaf and callus cell proliferation in broccoli.

Broccoli Brassica oleracea callus cell in vitro culture BAP IBA DPPH

References (14)

  1. 1 A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures [DOI]
  2. 2 Cost-effective in vitro propagation methods for pineapple [DOI]
  3. 3 Antioxidant potential and content of phenolic compounds in ethanolic extracts of selected parts of Andrographis paniculata [DOI]
  4. 4 Potential low-cost micropropagation of pineapple (Ananas comosus) [DOI]
  5. 5 Combination of the Auxins NAA, IBA, and IAA with GA3Improves the Commercial Seed-Tuber Production of Potato (Solanum tuberosumL.) underIn VitroConditions [DOI]
  6. 6 Plant regeneration by somatic embryogenesis and organogenesis in commercial pineapple (Ananas comosus L.) [DOI]
  7. 7 Somaclonal variation in micropropagated dormant axillary buds of pineapple (Ananas comosusL., Merr.) [DOI]
  8. 8 The Effect of Different Hormones and Incubation Periods on in vitro Proliferation of Pineapple (Ananas comosus L.) Merr cv. Smooth Cayenne) Shoot-Tip Culture [DOI]
  9. 9 Direct and Efficient Plant Regeneration from Different Explants Sources of Potato Cultivars as Influenced by Plant Growth Regulators [DOI]
  10. 10 Plantlet Production through Development of Competent Multiple Meristem Cultures from Male Inflorescence of Banana, Musa acuminta cv. ‘Pisang Mas’ (AA) [DOI]
  11. 11 In Vitro Propagation of Pineapple1 [DOI]
  12. 12 Piña Española Roja sin espinas propagada in vitro [DOI]
  13. 13 Potato (Solanum tuberosum L.) regeneration using the technique of meristem tip culture.
  14. 14 IMPROVEMENT AND PERSPECTIVES OF MICROPROPAGATION TECHNIQUES APPLIED TO SOME HOT CLIMATE PLANTS [DOI]

Cited by 2

EFFECT OF TEMPERATURE ON IN VITRO ORGANOGENESIS OF RHODIOLA IMBRICATA EDGEW. – A MEDICINAL HERB

Shubham Sharma · 2016

Showing 1 of 2 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

72

Page views

0

PDF downloads

69

Outbound clicks

2

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.