Skip to content
Research Article Open access CC BY 4.0

In-vitro Screening of Antimicrobial Activities of Ocimum gratissimum on Clinical Isolates

Ifeanyi Onyema Oshim, Evelyn Ukamaka Urama, Oluwayemisi Odeyemi, Augustina Nkechi Olise, Sunday Odeyemi

South Asian Journal of Research in Microbiology · pp. 1–7 · Published 12 Jun 2019

10.9734/sajrm/2019/v4i130095

Abstract

This study was undertaken to evaluate  the antimicrobial activities of crude  ethanol and methanol extracts of the leaves of Ocimum gratissimum L. (scent leaf) on Escherichia coli, Klebsiella  pneumoniae, Pseudomonas aeruginosa, Staphylococcus auerus and Candida albicans.The antimicrobial activities were carried out using  agar well diffusion method. The Minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC) of the plant extracts on the test isolates were determined by the agar dilution method. Ciprofloxacin and fluconazole (positive controls) were used in comparison with crude extract of O. gratissimum leaves and also, Dimethyl sulfoxide (DMSO) was used as the negative control. The ethanolic extract of O. gratissimum showed antibacterial activity with the mean inhibitory zone diameter of 3 -7mm against S. auerus, 2 mm against E. coli, 2 – 12 mm against K. pneumoniae, 2 mm against P.aeruginosa. Ethanol and methanol crude extracts of O. gratissimum leaves   showed no effect on   C.albicans. O. gratissimum extracts showed the lower   antimicrobial activity than the commercially available antibiotics (ciprofloxacin and fluconazole). The minimum Inhibitory Concentration and Minimum Bactericidal Concentration of the extracts on the test organisms also increased in the following order; methanol < ethanol. Hence, this   extract could   only serve   as antibacterial agent in the management of bacterial infection because it has no antifungal activities on Candida isolates used in this study.

Antibacterial agent clinical isolates Ocimum gratissimum agar well assay

References (12)

  1. 1 Antibiotic assay by agar-well diffusion method
  2. 2 DETERMINATION OF THERAPEUTIC POTENTIAL OF MENTHA LONGIFOLIA SSP LONGIFOLIA
  3. 3 Justification for the use of Ocimum gratissimum L in herbal medicine and its interaction with disc antibiotics [DOI]
  4. 4 Prevalence of hospital acquired enterococci infections in two primary-care hospitals in Osogbo, Southwestern Nigeria [DOI]
  5. 5 Phenolic content and biological activities of Rhus coriaria var. zebaria.
  6. 6 Antioxidant and Antimicrobial Activities of Salvia multicaulis [DOI]
  7. 7 Surgical Wound Infections [DOI]
  8. 8 Text Book of Pharmacognosy [DOI]
  9. 9 Kinetics of Minimum Inhibitory Concentration, Minimum Bactericidal Concentration and Minimum Fungicidal Concentration of Vernonia amygdalina (Bitter leaf) on Microorganisms Isolated from Wound Infections
  10. 10 Surgical wound infections [DOI]
  11. 11 Phytochemical Analysis and in-vitro Antimicrobial Activities of Crude Extracts of Aloe barbadensis (Aloevera) Leaf on Microorganisms Isolated from Surgical Wound Patients at Nnamdi Azikiwe University Teaching Hospital (NAUTH), South-Eastern, Nigeria
  12. 12 African Journal of Microbiology Research [DOI]

Cited by 4

Surfactant-Based Swollen Micelles Loaded with Oregano and Thyme Essential Oils as Aqueous Functional Ingredients: Colloidal Stability, Gastrointestinal Behavior, and Biological Activity

Agustina Delmonte, F. F. Visentini, Florencia M. M. Franco · Food Biophysics · 2026

Tithonia diversifolia Powder and Compost Ameliorate Plant Growth Parameters, Essential Oils, Phenols and Flavonoids Content and Anticandida Activity of Ocimum gratissimum L.

D. Blaise, Che Ruth Ngum, N. Edouard · European Journal of Medicinal Plants · 2022

Insecticidal activity and repellent potential of ocimum gratissimum on tribolium castaneum

Chiazom Onyedikachi Ezenwafor-Onyeocha, E. N. Nwankwo, I. Okwelogu · 2023

Showing 3 of 4 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

24

Page views

0

PDF downloads

3

Outbound clicks

4

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.