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In vitro and In vivo Antioxidant Capacity of Cowpea (Vigna unguiculata) Grown in Copper Polluted Soil

E. C. Onyeneke, O. J. Udele, E. Ehi-Imuse, M. U. Iyorzor

International Journal of Biochemistry Research & Review · pp. 43–57 · Published 6 Jul 2022

10.9734/ijbcrr/2022/v31i530324

Abstract

Vigna unguiculata is a drought tolerant legume which serves as a staple in sub-Sahara West Africa region especially in Nigeria. Studies have shown that cowpea possesses adaptive features which enables it to strive in dry regions. The present study investigates the antioxiodant capacity of the leaf and stem of cowpea (Vigna unguiculata) grown in copper polluted soil. Exactly 2kg of soil was treated with copper sulphate stock solution (1g/L) and kocide (a copper based pesticide) to obtain eight groups: 10mg/mL, 50mg/mL, 100mg/mL and 200mg/mL of copper sulphate, and 5.20g/L of kocide (lower dose of kocide), 6.67g/L of kocide (positive control) and 10.70g/L of kocide (higher dose of kocide), and no treatment in one group (negative control). The experimental plant, cowpea, was cultivated for five weeks. Data were collected after two weeks of cultivation and subsequent weeks until the fifth week, for the assays both on the homogenate and methanolic extract of the plant leaf and stem. The data obtained from the various assays conducted were subjected to analysis of variance (ANOVA) using Statistical Product and Service Solutions (SPSS) software (version 16). The results obtained showed that the malondialdehyde (MDA) level for both the leaf and stem showed an inconsistent increase (in lower dose of kocide, higher dose of kocide, 100mg/mL of copper sulphate and 200mg/mL of copper sulphate with 63.00 ± 8.82, 50.00 ± 5.77, 163.60 ± 3.33 and 157.00 ± 3.33 unit in mol/g wet weight × 10-4 respectively as against the negative control 73.00 ± 3.33MDA unit in mol/g wet weight × 10-4) within the weeks and decrease (in lower dose of kocide, higher dose of kocide, 100mg/mL and 200mg/mL with 17.00 ± 3.33, 23.00 ± 3.33, 17.00 ± 3.33 and 10.00 ± 0.00 unit in mol/g wet weight × 10-4 respectively as against the negative control 20.00 ± 5.77MDA unit in mol/g wet weight × 10-4) between weeks: depicting a stress condition in the plant. In the case of catalase, superoxide dismutase activities (SOD), total antioxidant capacity (TAC), ferric reducing antioxidant potential (FRAP), reducing power and nitric oxide (NO) free radical scavenging potentials; a consistent and significant increases within and between the weeks for both the leaf and stem were observed. The results obtained from the present study depict that Vigna unguiculata plant could tolerate a specific level of copper pollution (200 mg/mL) by enhancing the activities of enzymic antioxidants and other features.

Copper cowpea antioxidant capacity tolerance

References (33)

  1. 1 Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E [DOI]
  2. 2 Environmental science and pollution research [DOI]
  3. 3 [2] Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration [DOI]
  4. 4 Copper toxicity, oxidative stress, and antioxidant nutrients [DOI]
  5. 5 Heavy metal hyperaccumulating plants: How and why do they do it? And what makes them so interesting? [DOI]
  6. 6 Measurement of catalase activity in tissue extracts [DOI]
  7. 7 Elements of the Nature and Properties of Soils
  8. 8 Some Current Insights into Oxidative Stress [DOI]
  9. 9 Linking aboveground and belowground diversity [DOI]
  10. 10 Improving the production and utilization of cowpea as food and fodder [DOI]
  11. 11 Cowpea supply and demand in West and Central Africa [DOI]
  12. 12 Humus forms in terrestrial ecosystems: a framework to biodiversity [DOI]
  13. 13 [46] Antioxidant action of Ginkgo biloba extract EGb 761 [DOI]
  14. 14 Heavy Metal Stress and Crop Productivity [DOI]
  15. 15 A REVIEW ON ANTIOXIDANT METHODS [DOI]
  16. 16 The dynamics of heavy metals in plant–soil interactions [DOI]
  17. 17 Nanoscale copper in the soil–plant system – toxicity and underlying potential mechanisms [DOI]
  18. 18 Influence of plant species and phosphorus amendments on metal speciation and bioavailability in a smelter impacted soil: a case study of food-chain contamination [DOI]
  19. 19 In vitro trypanocidal effect of methanolic extract of some Nigerian savannah plants [DOI]
  20. 20 Changes of Ni biogeochemistry in the rhizosphere of the hyperaccumulator Thlaspi goesingense [DOI]
  21. 21 The influence of organic matter content of contaminated soils on the leaching rate of heavy metals [DOI]
  22. 22 Potential Ecological Risk and Health Risk Assessment of Heavy Metals and Metalloid in Soil around Xunyang Mining Areas [DOI]
  23. 23 Impact of nanoparticles and their ionic counterparts derived from heavy metals on the physiology of food crops [DOI]
  24. 24 Growth pattern of infants fed with a mixture of extruded malted maize and cowpea [DOI]
  25. 25 Enhanced Bioremediation of Heavy Metals from Phosphate Processing Wastewater Using the Indigenous BacteriumSerratia rubidaeaNCTC12971 [DOI]
  26. 26 Sustaining our soils and society.
  27. 27 Speciation and Mobility of Selected Heavy Metals in Sediments of the Nun River System, Bayelsa State, Nigeria
  28. 28 Effect of spilled engine oil on soil quality indicators and physiological performance of maize, cowpea and sorghum [DOI]
  29. 29 Plasma membrane NADPH oxidase and apoplastic POD can be responsible for accumulation of H2O2 in mesophyll cells of Elsholtzia haichowensis under copper stress [DOI]
  30. 30 Effect of different methods of Zn application on Zn, Fe, Cu and Mn concentration and uptake in some cowpea genotypes grown in mollisols of India
  31. 31 African Journal of Agricultural Research [DOI]
  32. 32 Bulletin of Environmental Contamination and Toxicology [DOI]
  33. 33 International Journal of Biology, Pharmacy and Allied Sciences [DOI]

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