Identification of Gibberellins and Cytokinins in Saladette Tomato Seeds

Abstract

Aims: Tomato (SolanumlycopersicumL.) is one of the most important vegetable crops worldwide, mainly as a result of its economic and nutritive contribution to human society. The presence of endogenous gibberellins and cytokinins in seeds of several crops has been related to a good germination; however, little is known in tomato. Therefore, the aim of this study was to analyze and identify the presence of gibberellins and cytokinins in saladette tomato seeds. Study Design: Laboratory analysis for gibberellins and cytokinins were conducted in solvent solutions groups with three technical replicates using a complete randomized design and results when applicable were analyzed using the statistical program 'RStudio' (version 10) and data obtained subjected to a comparison of means with the Tukey (P≤0.05) test. Place and Duration of Study: The experiment was conducted at the Department of Horticulture in Universidad AutónomaAgraria Antonio Narro, Saltillo, Mexico, during 2020-2021. Methodology: Lots of 50 grams dry weight of saladette “SVTE8832” tomato seed samples were freeze dried and prepared with several organic solvents for the extraction, purification and identification of gibberellins and cytokinins using the techniques of gas chromatography mass spectrometry(GC-MS) and gas chromatography mass spectrometry with selection ion monitoring(GCMS-SIM) respectively. Results: Gibberellins A1, A4, A7, A9, A12, A15, A17, A20, A44 and A53 were found in tomato seed tissue. The cytokininszeatin and zeatin-ribozide were also detected in analyzed tomato samples. Conclusion:The endogenous gibberellins A1, A4, A7, A9, A12, A15, A17, A20, A44, A53 and the cytokininszeatin and zeatin-riboside are present in saladette“SVTE8832” tomato seeds.