The Role of Factor X lll val 34 Leu Polymorphism in Intracerebral Hemorrhagic Stroke among Jordanian Patients: Molecular Analysis

Abstract

The polymorphism in the factor X lll A-submit gene (F X lll val 34 Leu) has been recongized as a risk factor for primary intracerebral hemorrahge (pich). In addition, F X lll valu 34 leu has a significant ethnic heterogenty. Objective: To study the possible role of a common mutation in the gene for coagulation factor X lll -A (val 34 Leu) as a risk factor for primary intracerebral hemorrhage stroke (pich) among Jordanian patients. Patients and Methods: A total thirty patients that have clinically suffered from cerebral hemorragic stroke, who are all aged between (40-62) years were entrolled in this study. DNA was extracted from plasma samples. Genotyping of F X lll val 34 Leu polymorphism was detected by a 192 bp, PCR-technique and the PCR product was digested with Ddel restriction enzyme.   Results: In this study two bands in fragment length (192 bp and (161 bp) were found in seven patients as positive results 25% the other patients showed one fragment of 192 bp as negative results (75%). Conclusion:  These results suggested that factor Xlll val 34 Leu has a role in the pathogenesis primary intracerebral hemorrhage (pich), this may be related to impaired cross-linking of fibrin.