Preparation of Protein Extraction from Flower Buds of Solanum lycopersicum for Two-Dimensional Gel Electrophoresis
Xiaocui Zhao, Jingqi Ren, Na Cui, Haiyan Fan, Guangchao Yu, Tianlai Li
Biotechnology Journal International · pp. 183–190 · Published 23 Mar 2013
10.9734/BBJ/2013/2957Abstract
Aims: Find a suitable method for the protein extraction from flower buds of Solanum lycopersicum. Study Design: Compare some kinds of protein extraction methods and find the best one among them suitable to tomato flower buds. Place and Duration of Study: Biological Science and Technology College, between June 2010 and July 2011. Methodology: The proteins for electrophoresis were extracted using different methods, such as trichloroacetic acid /acetone (TCA/acetone), Sodium dodecyl sulfate (SDS), Tris-saturated phenol (Tris-Phen), Phenol/SDS and Direct lysis method. After silver staining, different patterns of protein spots were observed in the gels. Results: Few spots were found by SDS and Phenol/SDS extractions, more spots by immediate dissolution but the most impurities, less protein productivity though more spots by Tris-Phen extractions, and more protein productivity and better apart effect by TCA/acetone. The 2-DE image background was the clear and the protein spots were the most by TCA/acetone method. Conclusion: TCA/acetone method is much more suitable as extraction method for protein two-dimensional electrophoresis of tomato flower buds.
Cited by 1
Sophie Alvarez, Michael J. Naldrett · Advances in Experimental Medicine and Biology · 2016
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