Comparison of Blood and Urine Profiles of Remogliflozin in Rat PK Study Using a Single and Simple UPLC Method
G. K. Aiswarya, P. C. Indukala, S. Kathirvel, K. Raja Rajeswari
Asian Journal of Research in Nephrology · pp. 53–63 · Published 13 May 2023
Abstract
This study describes a comparison between blood and urine profiles of Remogliflozin simultaneously in a rat pharmacokinetic study using a simple UPLC method. Quantitative determination of Remogliflozin is performed in rat blood with the recovery values in the range of 85-115%. A reversed phase Ultra-performance liquid chromatography (UPLC) with UV detector is used. The method utilizes an Acquity UPLC CSH Fluoro-Phenyl column (150x1mm, 1.7 µm) and a gradient elution with mobile phase consisting of acetonitrile and 0.1% formic acid (15:85) at a flow rate of 0.8 mL min-1. The method adequately resolves the analyte and internal standard (metformin) within a run time of 5 min. The UV detection carried out at 275 nm. In both blood and urine, the detection limit of Remogliflozin is found to be 2 µg mL-1 and the LOQ as 10 µg mL-1. Two profiles were compared by conducting single oral dose in vivo rat PK study after complete validation of the UPLC method.
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