Effect of Mexican Oregano (Lippia graveolens Kunth) on Streptozotocin Induced Diabetic Mice and its Role in Regulating Carbohydrate Metabolic Enzymes and Their inhibitory Effect on the Formation of Advanced Glycation end Products

Abstract

Aim of the Study: The aim was to evaluate the effect of extracts of the leaves of Lippia graveolens in streptozotocin-induced mildly diabetic (MD) and severely diabetic (SD) miceand on the formation of advanced glycation end products (AGEs). Study Design: Diabetes has emerged as a major global challenge in healthcare delivery, particularly in recent times, with the global incidence reaching epidemic proportions. Because of this there is an increasing demand to research for natural products with antidiabetic activity. Place and Duration of Study: Laboratory of Natural Products Research. School of chemical engineering, National Polytechnic Institute between August 2013 and April 2014. Methodology: Extracts were orally administered to MD and SD rats for 30 days, and a set of biochemical parameters were studied: glucose, cholesterol, triglycerides, lipid peroxidation, glycogen, SOD, CAT, GSH, GPX, glucose-6-phosphatase, glucokinase, hexokinase activities, SGOT, SGPT, ALP and insulin level. In this study, we investigate the inhibitory effects In vitro on the formation of specific AGE representatives including AGEs-BSA formation, Amadorin activity,methylglyoxal (MGO). In vivo was to investigate the effect of extracts on oxidative stress, glycation of hemoglobin and MGO, glycolaldehyde (GA) levels, TBA-reactive substance level in kidney mitochondrial instreptozotocin-induced diabetic mice. Results: Methanol extract (LG-M) reduced the intake of food, water and body weight loss as well as levels of blood glucose, serum cholesterol, triglyceride, lipoprotein and increase HDL-cholesterol, antioxidant enzymes, improves TBARS–reactive substance, marker enzymes of hepatic function. These results, support that improves glucose metabolism by reducing insulin resistance. LG-M is an inhibitor of fluorescent AGE, methylglyoxal and the glycation of haemoglobin. Conclusions: This study demonstrated that Lippia graveolens possesses considerable anti-AGE and hepatoprotective role, inhibits hyperglycemic, hyperlipidemic and oxidative stress indicating that these effects may be mediated by interacting with multiple target operating in diabetes mellitus.