Genotoxic and Cytotoxicity Activities of Leaf Extract of Setaria megaphylla
Okokon J. E., Ochigbo E. B., Johnny I. I., Anagboso M. O., Ebong N. O.
Asian Journal of Biochemistry, Genetics and Molecular Biology · pp. 56–64 · Published 20 Oct 2023
10.9734/ajbgmb/2023/v15i3339Abstract
Setaria megaphylla a medicinal plant, used in ethnomedicine for the treatment of malaria, diabetes and inflammatory diseases was investigated for cytotoxic and genotoxic effects on cells of the root meristem of Allium cepa. Bulbs of Onion were exposed to 2.5 mg/ml, 5mg/ml, and 10 mg/ml concentrations of the leaf extract for macroscopic and microscopic study. Tap water was used as a negative control and Methotrexate (0.1 mg/ml) as a positive control. Depending on concentration by the extract, there was statistically significant (P < .05) inhibition of root growth when compared with the negative control group. All the tested concentrations of the extract were observed to have cytotoxic effects on cell division in A. cepa. The extract- induced micronuclei (MNC) formations and chromosomal aberrations in A. cepa root tip cells were significant (P < .05) when compared with control group. The extract treatment further induced, ghost cells, cells membrane damage, cell death, and bi nucleated cells. These results implies that Setaria megaphylla leaf extract possess cytotoxic and genotoxic effects on A. cepa which is as a results of its phytochemical constituents.
References (12)
- 1 IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans [DOI]
- 2 In vivo rodent micronucleus assay: protocol, conduct and data interpretation [DOI]
- 3 Mitotic Abnormalities Induced by Silk Dyeing Industry Effluents in the Cells of Allium cepa. [DOI]
- 4 Microtubule dynamics in interphase cells. [DOI]
- 5 Recommended protocols based on a survey of current practice in genotoxicity testing laboratories, IV. Chromosome aberration and sister-chromatid exchange in Chinese hamster ovary, V79 Chinese hamster lung and human lymphocyte cultures [DOI]
- 6 Cytotoxic and genotoxic effects of Lavandula stoechas aqueous extracts [DOI]
- 7 Cytological Effect of the Potassium Metabisulphite and Potassium Nitrate Food Preservative on Root Tips of Allium cepa L. [DOI]
- 8 In vitro antiplasmodial activity of some medicinal plants from Nigeria
- 9 The "Allium" Test: A Simple, Eukaryote Genotoxicity Assay [DOI]
- 10 Anti-mitotic and anti-genotoxic effects of Plantago lanceolata aqueous extract on Allium cepa root tip meristem cells [DOI]
- 11 Anti-inflammatory and anti-nociceptive effects of ethanolic extract of Setaria megaphylla leaves in rodents [DOI]
- 12 Hypoglycaemic and Antidiabetic Effect of Setaria megaphylla on Normal and Alloxan Induced Diabetic Rats [DOI]
Cited by 10
Emmanuella Chinyere Ikechukwu, Peace Nwachukwu Agu, Hephzibah Boluwatito Olumuji, Martin Osita Anagboso, Imoh Imeh Johnny, Jude Efiom Okokon, Nwakaego Omonigho Ebong · Asian Journal of Biochemistry, Genetics and Molecular Biology · 2024
Ugonma Florence Uwaeme, Chinyelu Clementina Osigwe, Jude Efiom Okokon · Journal of Biomolecular Toxicology · 2025
Chinyelu Clementina Osigwe, Ugonma Florence Uwaeme, Jude Efiom Okokon · Journal of Biomolecular Toxicology · 2025
Chinyelu Clementina Osigwe, Jude Efiom Okokon, Ukeagbu Wilfred · Trends in Natural Products Research · 2025
Rose Winny Ebong Eyango, Landry Lienou Lienou, Marie Stephanie Chekem Goka · Journal of Herbal Medicine · 2025
Showing 5 of 10 known citations — external sources report more than can currently be individually listed.
Related research
- Comparative Studies on Anti-Inflammatory, Antioxidant and Antimutagenic Activities of Crassocephalum crepidioides (Bent) Leaf Cold and Hot Water Extracts — shares topic coverage
- Genotoxicity of Methyl Tert-butyl Ether (MTBE) to Vicia faba L. Plants — shares topic coverage
- Cadmium Induced Toxicity in Human Peripheral Blood Lymphocytes Culture and Its Amelioration by Vitamin C — shares topic coverage
- In vitro Cytotoxic and Genotoxic Effects of Methanol and Aqueous Extracts of Gymnosporia Montana Plant — shares topic coverage
- Chemoprotective Effect of Eugenol on Repeated dose Doxorubicin, Induced Genotoxic Lesions in Mouse Bone Marrow — shares topic coverage
Article metrics
Real usage data collected on this platform.
48
Page views
0
PDF downloads
19
Outbound clicks
10
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.