Genetic Diversity Analysis in Mungbean (Vigna radiata L.) Genotypes Using SSR Markers
MD Afroz, Premakumar, Imatiyazahamed Teli, Hima Kumar Anakali, V Thirumala Rao, Y Hari, V Sridhar
Journal of Advances in Biology & Biotechnology · pp. 1842–1848 · Published 24 Oct 2025
10.9734/jabb/2025/v28i103196Abstract
Background: Nineteen mungbean [Vigna radiata (L.) Wilczek] genotypes were assessed for molecular diversity using simple sequence repeat (SSR) markers. Methods: A total of 26 SSR primer pairs were employed to evaluate genetic variation among the genotypes. The amplified products were scored in a binary format, and similarity coefficients were calculated using Jaccard’s method in NTSYS-pc version 2.1. Using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA), a dendrogram was produced. Results: Among the 26 SSR primers screened, eleven exhibited polymorphism, while thirteen were monomorphic and two did not yield any amplification. Genetic similarity coefficients ranged from 0.22 to 1.00, with an average of 0.61. The genotypes SML-668 and MGG-551 showed complete genetic similarity (1.00), whereas Tarm-18 and GP-2-57 were the most divergent (0.30). These results suggest that SSR markers are effective tools for distinguishing mungbean genotypes and for identifying suitable parental combinations for breeding programs.
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