Gene Editing in Cancer Immunotherapy: Mechanisms, Advancements, Limitations and Future Directions
Lawrence John AJutor, Okabeonye Sunday Agbo, Christian Ajiri Adobor, Blessing Aitebiereme Iyoyojie, Kingsley Ugonna Ugoagwu, Oghenevware Manawa, Osaruese Faluyi, Emmanuel Oluwasegun Ismaila, Oluchi Esther Ugwuanyi, Adepeju Matilda Adekoya
Asian Journal of Biochemistry, Genetics and Molecular Biology · pp. 121–135 · Published 21 Dec 2024
10.9734/ajbgmb/2024/v16i12428Abstract
Gene editing has emerged as a transformative approach in cancer immunotherapy. Several gene editing tools have been employed for precise modification of the DNA of immune cells, enhancing their ability to target and eliminate cancer cells. This review examines the evolution and applications of key gene-editing tools, such as CRISPR-Cas9, TALENs, ZFNs, and recent innovations like base and prime editing in the field of cancer immunotherapy. Promising results have been observed in therapies such as CAR-T and tumor-infiltrating lymphocyte (TIL) treatments, which have shown success in cancers like leukemia and lymphoma. These technologies improve immune system function by disrupting checkpoints, boosting cytokine production, and modifying tumor microenvironments. Significant clinical trials have demonstrated promising outcomes, such as CRISPR-Cas9-engineered T cells targeting refractory cancers, which showed improved efficacy and safety. However, despite these advancements, there have been limitations, including off-target effects, delivery inefficiencies, immunogenicity, and ethical concerns, alongside the high costs that hinder widespread adoption. Future directions for gene editing in cancer therapy include the integration of AI and machine learning to enhance target accuracy and guide RNA design, as well as novel gene-editing systems such as the I-C Cascade-Cas3, which facilitates large-scale genomic deletions. Furthermore, CRISPR-based epigenome editing holds promise for further advancing cancer therapies. These innovations, combined with optimized delivery methods, are expected to improve the precision, efficacy, and accessibility of gene editing in cancer immunotherapy.
References (36)
- 1 CRISPR-engineered T cells in patients with refractory cancer [DOI]
- 2 Engineering CAR-T Cells for Next-Generation Cancer Therapy [DOI]
- 3 CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy [DOI]
- 4 Origins and evolution of CRISPR-Cas systems [DOI]
- 5 Current applications and future perspective of CRISPR/Cas9 gene editing in cancer [DOI]
- 6 CRISPR-Cas9 DNA Base-Editing and Prime-Editing [DOI]
- 7 MHC Class I Downregulation in Cancer: Underlying Mechanisms and Potential Targets for Cancer Immunotherapy [DOI]
- 8 Gene Therapy for Cancer Treatment: Past, Present and Future [DOI]
- 9 Mechanisms of Cancer Resistance to Immunotherapy [DOI]
- 10 A Review on the Mechanism and Applications of CRISPR/Cas9/Cas12/Cas13/Cas14 Proteins Utilized for Genome Engineering [DOI]
- 11 CRISPR technologies for genome, epigenome and transcriptome editing [DOI]
- 12 Epigenome editing technologies for discovery and medicine [DOI]
- 13 Mechanisms of tumor resistance to immune checkpoint blockade and combination strategies to overcome resistance [DOI]
- 14 The interaction of intestinal microbiota and innate lymphoid cells in health and disease throughout life [DOI]
- 15 Efficient Gene Editing at Major CFTR Mutation Loci [DOI]
- 16 CRISPR/Cas9 Genome Editing for Tissue‐Specific In Vivo Targeting: Nanomaterials and Translational Perspective [DOI]
- 17 Precision RNA base editing with engineered and endogenous effectors [DOI]
- 18 Delivery of CRISPR/Cas9 system by AAV as vectors for gene therapy [DOI]
- 19 CRISPR/Cas9 in Cancer Immunotherapy: Animal Models and Human Clinical Trials [DOI]
- 20 CRISPR Craze to Transform Cardiac Biology [DOI]
- 21 Genome Editing in Engineered T Cells for Cancer Immunotherapy [DOI]
- 22 Calponin 3 suppresses proliferation, migration and invasion of non‑small cell lung cancer cells [DOI]
- 23 Clinical trials and promising preclinical applications of CRISPR/Cas gene editing [DOI]
- 24 An Overview of Tools for Genome Editing: ZFNs, Mega Nucleases, and TALENs [DOI]
- 25 Current Advances and Material Innovations in the Search for Novel Treatments of Phenylketonuria [DOI]
- 26 The CRISPR-Cas System and Clinical Applications of CRISPR-Based Gene Editing in Hematology with a Focus on Inherited Germline Predisposition to Hematologic Malignancies [DOI]
- 27 Emerging Gene-editing nano-therapeutics for Cancer [DOI]
- 28 In vivo application of base and prime editing to treat inherited retinal diseases [DOI]
- 29 Therapeutic potential of CRISPR/CAS9 genome modification in T cell-based immunotherapy of cancer [DOI]
- 30 Zinc Finger Nuclease (ZFNs) and Transcription Activator-like Effector Nucleases (TALENs) Based Genome Editing in Enhancement of Anticancer Activity of Plants [DOI]
- 31 Clinical gene therapy development for the central nervous system: Candidates and challenges for AAVs [DOI]
- 32 Transcription Activator-Like Effector Nucleases (TALENs): Genome Editing Tool to Explore Enhanced Activity of Antidiabetic Plants [DOI]
- 33 Allogeneic Chimeric Antigen Receptor T Cells: A Further Potential Weapon for Haematological Malignancies [DOI]
- 34 Science [DOI]
- 35 ALLO-647 for Lymphodepletion in the Allogeneic CAR T Setting: Safety Experience with ALLO-501/501A in Patients (Pts) with Relapsed/Refractory (r/r) Large B-Cell and Follicular Lymphomas [DOI]
- 36 CRISPR-Cas12/Cas13: bibliometric analysis and systematic review of its application in infectious diseasedetection [DOI]
Cited by 7
Lawrence John AJutor, Fatima Bello Sani, Elijah Nehemiah Kama, Kingsley Ugonna Ugoagwu, Adegbesan Abiodun Christopher, Daniel Temiloluwa Oyedemi, Peter Bemigho Adewoyin, Olayinka Esther Agbetanmije, Adepeju Matilda Adekoya, Erere Godsent Onyeugbo · Journal of Cancer and Tumor International · 2025
Dilpreet Singh, Lalu Muhammad Irham · AAPS PharmSciTech · 2025
Bakr Ahmed Taha, Ali J. Addie, Adawiya J. Haider · Gene Therapy · 2026
Chetana Krushna Belkare, Omkar Vishnu Daware · Drug Development and Industrial Pharmacy · 2026
Showing 4 of 7 known citations — external sources report more than can currently be individually listed.
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