Cardioprotective Effect of Portulaca Oleracea L. (Purslane) against Clozapine-Induced Cardiotoxicity in Rats

Abstract

Aim: To investigate the protective role of purslane (regla) on clozapine-induced toxicity in rats. Methods: Fifty male Sprague–Dawley rats were divided into five groups (10 rats per group) as follows: Group1 (control group): Rats injected daily with buffered saline for 21 days. Group2 (cardiotoxic group): Rats injected with clozapine (15mg/kg body weight) for 21 days. Group3: Rats injected daily with clozapine (15mg/kg body weight) for 21 days + 10% of regla water seed extract. Group4: Rats injected daily with clozapine (15mg/kg body weight) for 21 days + 10% of regla dry leaves water extract. Group5: Rats injected daily with clozapine (15mg/kg body weight) for 21 days + 10% of regla juice. Results: Serum marker enzymes as creatinine kinase, lactate dehydrogenase, AST and troponin T were estimated, as well as inflammatory markers; E-selectin, myeloperoxidase (MPO) and CRP. Caspase-3 and MDA were also estimated. The result showed that purslane seeds, dry leaves extract or juice have ameliorative effect on clozapine-induced cardiac toxicity in rats through decreasing serum CK, LDH, AST and Troponin enzyme activities, serum E-selectin, CRP, myeloperoxidase, caspase-3 and MDA levels compared to cardiotoxic group with purslane juice being the most effective treatment. Conclusion: It can be concluded that purslane juice, dry water extract or seeds extract can be used for the protection of clozapine induced toxicity.