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Research Article Open access CC BY 4.0

Sub Acute Toxicity Study of Mixed Fungal Culture Filtrates Isolated from Maize Stalks of Shivamogga District in Rats

Manjunath Uppar, Vinay P. Tikare, N. B. Shridhar, U. Sunilchandra, P. Ravikumar, K. Nagaraja

Microbiology Research Journal International · pp. 244–254 · Published 29 Dec 2024

10.9734/mrji/2024/v34i121525

Abstract

Aim: To assess effects of the toxicity caused by consuming the fungal contaminated fodder by the cattle in the field conditions. Study Design: Study was conducted in 3 groups of Wistar Albino rats. Place and Duration of Study: Department of Veterinary Pharmacology and Toxicology and Department of Veterinary Pathology, Veterinary College Shivamogga, Karnataka, between August 2024 to October 2024. Methodology: A repeated-dose 28-days oral sub acute toxicity study was performed with three groups (n=6) of Wistar Albino rats. The group I (control) received oral gavage of potato dextrose broth @ dose of 2 mL/100g, where as groups II and III received mixed fungal culture filtrate of Aspergillus flavus and Aspergillus niger at the doses of 1 and 2 mL/100 g respectively. Results: Treated groups (group II and III) showed increase in serological parameters (Aspartate Transaminase, Alanine transaminase, Creatinine, Blood urea nitrogen) and decrease in hematological parameters (PCV, Hb, TLC, TEC). Conclusion: The findings of the present study inferred that the fungal culture filtrates of fungus isolated from contaminated maize stalk caused toxicity in rats, which was attributed for the presence of a toxic principle components in the fungal culture filtrates.

Aspergillus flavus Aspergillus niger toxicity study rats

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