Transferability of Expressed Sequence Tag of Single Sequence Repeats Markers of Tomato Fruit in Different Vegetable Varieties

Abstract

Aims: The expressed sequence tag of single sequence repeats (EST-SSR) primers designed from tomato fruit were studied for their polymorphism and transferability to other vegetable varieties. Study Design: Primers were designed from ESTs in which the repeat bases of SSR were more than 18 bp. Twenty primer pairs chosen randomly were tested for their ability to amplify among 14 tomato varieties. The primer pairs were further used for 8 Chinese cabbage varieties, 8 muskmelon varieties and 8 eggplant varieties. Place and Duration of Study: College of Biological Science and Technology, Shenyang Agricultural University, China, between February 2015 and March 2016. Methodology: Twenty primer pairs selected randomly were used for the amplification of genomic DNA in 14 tomato varieties. Genomic DNA of tomato was isolated and amplified, and tested the transferability of primers. Results: Eighteen of primer pairs showed the amplification, and 2 primer pairs showed the non-pre-amplification. Seventeen of 18 primer pairs revealed polymorphism. Eighteen primer pairs were further used for PCR to genomic DNA from 8 Chinese cabbage varieties, 8 muskmelon varieties and 8 eggplant varieties. Twelve of 18 primer pairs showed amplification in all of materials provided, and the individual ratios of amplification were 77.8%, 83.3% and 83.3%, the ratios of polymorphism of them were 65.0%, 46.7% and 80%, respectively. Conclusion: Eighteen primer pairs showed the amplification. Seventeen of 18 primer pairs revealed polymorphism. Twelve of 18 primer pairs showed amplification in 8 Chinese cabbage varieties, 8 muskmelon varieties and 8 eggplant varieties. Tomato EST-SSR markers had highly transferability to other plant species.