The Potential Role of Ki-67 Index in the Prognosis of Breast Cancer and Its Relation to Molecular Subtypes in Egyptian Females with Breast Cancer

Abstract

Background: Breast cancer (BC) is a well-documented major cause of female morbidity and mortality worldwide. Ongoing research era is focusing on the establishment of diagnostic and prognostic markers, helping for early pick up of the cases, proper prognosis evaluation and clarifying reliable treatment strategy. Aim of the Study: This study aimed to evaluate the role of  Ki-67 as prognostic marker for breast cancer  in Egyptian females population. Patients and Methods: 120 BC patients and 30 age and BMI matching health controls are the subjects of the study, Ki-67 index values were investigated by immunohistochemistry that was performed on 5-lm slides of formalin-fixed and paraffin-embedded archival tumor tissue (core needle biopsy samples). Antigen retrieval was performed in a micro-oven in citrate buffer pH 6 for 20 minutes. Ki-67–stained slides were captured digitally at a hot spot at 3200 magnification. The Ki-67 labeling index was measured using digital image analysis software. Image analysis was performed by an experienced pathologist. Ki-67 index values were correlated with the clinicopathologic aspects of the BC patients. Results: Our study showed that Ki-67 index values revealed gradual increase with disease severity and correlated with poor prognosis aspects. Conclusion: Ki-67 index values are shown to be associated with breast cancer prognosis,  supporting their role as prognostic biomarkers.