Skip to content
Research Article Open access CC BY 4.0

Fast and Easy Protocol of DNA Extraction from Fresh and Hard/Dried Plant Samples of Indian Spices

Sudeep Nagaraj, Ragunandana Girenahalli, Praveen Kumar Kondenahalli Subbarayappa, Jagadish Tavarekere Venkataravanappa

International Journal of Plant & Soil Science · pp. 1–9 · Published 21 Sep 2018

10.9734/IJPSS/2018/44061

Abstract

Spices identified using basic techniques like magnifying glasses or microscopic instruments. The DNA based spice detection techniques used in food testing labs for the disclosure of adulterants. The molecular techniques like PCR, Sequencing, and HRM etc. enables the exact disclosure of spices. Plants synthesize more amounts of polysaccharides, flavonoids, polyphenols and other secondary metabolites which interfere with the pure and high-quality genomic DNA extraction. Although plant DNA isolation protocols, extracting DNA from dried plant samples is a challenging task. This present research describes an expeditious, reliable SDS buffer protocol suited for extracting DNA from both fresh and dried plants samples rich in polysaccharides and secondary metabolites, and the protocol does not need toxic chemicals like phenols and use of liquid nitrogen. Extracted DNA showed the excellent purity evidenced by UV spectrometer at the ratio of A260/A280 ranging from approximately 1.8-1.85 and the A260/A230 ratio was approximately 2, which explains the preparations are sufficiently free from proteins and polysaccharides. The developed method is satisfactory for both dry and fresh spice samples. The success of the present study is getting high-quality genomic DNA and helps to carry out DNA based experiments with a shorter duration of time.                                                                                                                         

SDS DNA isolation corrosive chemicals dry and fresh plant samples

References (9)

  1. 1 Principles and Techniques of Biochemistry and Molecular Biology [DOI]
  2. 2 An Effective Method of DNA Isolation from the Mature Leaves of Gossypium Species That Contain Large Amounts of Phenolic Terpenoids and Tannins [DOI]
  3. 3 A simplified universal genomic DNA extraction protocol suitable for PCR [DOI]
  4. 4 Extremely conserved histone H4 N terminus is dispensable for growth but essential for repressing the silent mating loci in yeast [DOI]
  5. 5 Isolation of milligram quantities of nuclear DNA from tomato (Lycopersicon esculentum), A plant containing high levels of polyphenolic compounds [DOI]
  6. 6 Extraction of DNA suitable for PCR applications from mature leaves of Mangifera indica L. [DOI]
  7. 7 A rapid and hazardous reagent free protocol for genomic DNA extraction suitable for genetic studies in plants [DOI]
  8. 8 A rapid method for high throughput DNA extraction from plant material for PCR amplification [DOI]
  9. 9 Nucleic Acids Research Group (NRG): The Importance of DNA Extraction in Metagenomics: The Gatekeeper to Accurate Results!

Cited by 0

No indexed citations yet.

Related research

Article metrics

Real usage data collected on this platform.

55

Page views

0

PDF downloads

18

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.