Sudeep Nagaraj

Separation and Optimisation of a Sucrose Density Gradient Centrifugation Protocol for Isolation of Peripheral Blood Mononuclear Cells (PBMC)

Sudeep Nagaraj, Shubha Nivargi, Leelavathy Nanjappa & Jagadish Tavarekere Venkataravanappa · Asian Journal of Research in Biochemistry · 2018

One step centrifugation procedure used commonly for separation of blood cells is the ficoll gradient centrifugation. In this method, after centrifugation, the peripheral blood mononuclear cells (PBMCs) are located on the top of the separation fluid, whereas other blood cells eryt...

Open access Research Article 10.9734/ajrb/2018/v3i229820

Fast and Easy Protocol of DNA Extraction from Fresh and Hard/Dried Plant Samples of Indian Spices

Sudeep Nagaraj, Ragunandana Girenahalli, Praveen Kumar Kondenahalli Subbarayappa & Jagadish Tavarekere Venkataravanappa · International Journal of Plant & Soil Science · 2018

Spices identified using basic techniques like magnifying glasses or microscopic instruments. The DNA based spice detection techniques used in food testing labs for the disclosure of adulterants. The molecular techniques like PCR, Sequencing, and HRM etc. enables the exact disclos...

Open access Research Article 10.9734/IJPSS/2018/44061