Skip to content
Research Article Open access CC BY 4.0

Evaluation of Hepatoprotective Activity and Acute Toxicity of Guiera Senegalensis Leaf Extract on Wistar Rats

Mas’-ûd Hayatou, Estella Achick Tembe, Bayaga Herve, Njinkio Nono Borgia, Charles N. Fokunang

Journal of Advances in Medical and Pharmaceutical Sciences · pp. 1–17 · Published 14 Mar 2024

10.9734/jamps/2024/v26i5682

Abstract

A liver is an essential organ that performs several metabolic activities in body. Various chemicals as well as pathologies which affecting it result in harmful consequences. Management of liver pathologies involves expensive and even toxic therapeutic strategies. 80 % by populations in developing countries used medicinal plants which constituted a significant reservoir of molecules with diverse and various properties including hepatoprotective properties. To rectify these problems, Guiera senegalensis has been in existence that subjected to few studies and highlighted hepatoprotective properties, hence the interest of this study. Three extraction methods were carried out, infusion, decoction, maceration (aqueous, ethanolic). The hepatoprotective activity of the extracts was explored on 42 Wistar rats divided into 7 groups of 6 rats each. The groups including 4 test groups, were treated with the extract at 500 mg/kg of each extract. A healthy group that received distilled water (positive control group) were treated with paracetamol at 640mg/Kg. A reference group received a drug (Silymarin) The rats were treated for 14 days. The rats were then sacrificed, blood sample were collected for biochemical analyses. Some organs were removed for histological analysis as well. The acute toxicity of the extract obtained by hydro-ethanolic maceration at a dose of 2000 mg/kg was conducted in accordance with OECD guideline 420. The study of the hepatoprotective activity of plant extracts showed a significant decrease in transaminase activities in the rats treated with the extract obtained by aqueous maceration, AP, a significant decrease in total proteins in the extract obtained by hydro-ethanolic maceration. It was revealed that, the increase in in-vivo antioxidants found to be high in those treated with the hydro-ethanolic extract. The hydro-ethanolic extract did not show any significant toxicity.

Guiera senegalensis hepatoprotection leaf extract wistar rats

References (19)

  1. 1 Tissue sulfhydryl groups [DOI]
  2. 2 Colorimetric assay of catalase [DOI]
  3. 3 Quantitative Determination of Serum Triglycerides by the Use of Enzymes [DOI]
  4. 4 Albumin standards and the measurement of serum albumin with bromcresol green [DOI]
  5. 5 The thiobarbituric acid reagent as a test for the oxidation of unsaturated fatty acids by various agents.
  6. 6 In vivo antioxidant activity of phenolic compounds: Facts and gaps [DOI]
  7. 7 Test No. 420: Acute Oral Toxicity - Fixed Dose Procedure [DOI]
  8. 8 Molecular Biomarkers in Drug-Induced Liver Injury: Challenges and Future Perspectives [DOI]
  9. 9 Pharmacotherapies for Drug-Induced Liver Injury: A Current Literature Review [DOI]
  10. 10 Malondialdehyde (MDA) – product of lipid peroxidation as marker of homeostasis disorders and aging [DOI]
  11. 11 Effects of nerol on paracetamol-induced liver damage in Wistar albino rats [DOI]
  12. 12 Mechanism of drug-induced liver injury and hepatoprotective effects of natural drugs [DOI]
  13. 13 An Overview of Methods of Extraction, Isolation and Characterization of Natural Medicinal Plant Products in Improved Traditional Medicine Research [DOI]
  14. 14 Worldwide Importance of Medicinal Plants: Current and Historical Perspectives [DOI]
  15. 15 Importance of Medicinal Plants in Human and Plant Pathology: A Review [DOI]
  16. 16 Aspartate aminotransferase. [DOI]
  17. 17 Biochemical and Toxicological Activity of Guiera senegalensis Leaves Extract [DOI]
  18. 18 Effect of Guiera senegalensis and Natron on Serum Indices of Cardiac Function of Postpartum Rats [DOI]
  19. 19 Organizational Principles of the Liver [DOI]

Cited by 4

Advances in mesenchymal stem cell therapy and natural antioxidants for hepatic fibrosis: A comprehensive review

Abeer Kazmi, Tahira Sultana · Pharmacological Research - Reports · 2024

Showing 1 of 4 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

25

Page views

0

PDF downloads

3

Outbound clicks

4

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.