Evaluation of Antimicrobial Activity of Commiphora myrrh against Standard Bacterial Strains and Clinical Isolates with Chemical Analysis Profiling

Abstract

Aims: The objective of present study was to investigate the chemical analysis biological activates of commiphora myrrh the chemical analysis of myrrh was analyzed several ways. Study Design: Where wet digestion was used to estimate the concentrations of a number of chemical elements in it, which are of great importance to humans and are also attributed to many of its medicinal uses. Place and Duration of Study:  Clinical isolates from wound infections obtained from laboratory of Marjan Hospital Hilla city Iraq during period Fed. 2020 which include (four  E. coli  and  4  of  S. aureus,  and 4 pseudomonas aeruginosa ). All clinical isolates were classified and by laboratory of marjan hospital: Hilla city Iraq. Methodology: In order to know the nature of the groups present in it, in addition to the quality of the organic materials, FTIR analysis and G.C analysis were used by using the ethanolic extract, where some of the organic materials within their compositions in identified. Results: The antimicrobial potential of ethanolic extracts of myrrh were studied against many standard strains of gram positive and gram negative bacteria and (12) clinical isolated from patients with wound infections obtained from the bacteriology section of the clinical microbiology laboratory of marjan hospital Hill city/Iraq during period Feb. 2020 to Nov. 2020. The clinical isolates include (4) isolates of staphylococcus aureus, (4) isolates of E.coli and (4) isolates of pseudomonas aeruginosa, and it was confirmed using the usual methods of diagnosis. The broth dilution method was used for determination of the MICs of (minimal inhibitory concentration) of myrrh extract against pathogens under study. Six concentration (80, 60, 30, 12, 6, 3 mg/mL) of myrrh extracts were tested. Conclusion: The result revealed that the highest activity was against S. Aureus at concentration (80, 60, 30 mg/mL) which showed completely inhibition of the growth (100%). While the gram-negative bacteria E.coli and P. auroginosa the concentration (80 – 60 mg/mL) showed 100% inhibition in contrast the concentration (12, 6, 3 mg/mL) showed no activity of myrrh extract against all pathogens under study. The result indicates that myrrh is an antibacterial agent that can be used in the future by making appropriate doses.