Evaluating the Effect of Chloroform Inhalation as a Method of Euthanasia on the Cerebellum and Hippocampus of Adult Wistar Rats
Ugochukwu Samuel Aguwa, Okeke Somadina Nnamdi, Ezejindu Darmian Nnabuihe, Eze Chinyere Elizabeth, Azurunwa Ogechi, Obinwa Benedict Nzube, Ovie Ogbo. Felix, Obi Kelvin Chukwuemeka, Onwuelingo Sopuru, Okonkwo David, Doris Ogbuokiri, Okeke Chijioke
Journal of Advances in Medical and Pharmaceutical Sciences · pp. 14–25 · Published 27 Jul 2020
10.9734/jamps/2020/v22i630176Abstract
Chloroform inhalation is a common method of rodent euthanasia in Nigeria for research purposes. This work is designed to evaluate the consequence of this method of sacrifice on the cerebellum and hippocampus of Wistar rats. Twenty male Wistar rats weighing between 160 and 180 were divided into 4 groups of 5 rats each. Group A served as control and was sacrificed using cervical dislocation (a widely acceptable non-inhalation method of sacrifice). Groups B, C and D were exposed to chloroform for 5 minutes once a day (group B), once a day for 2 days and once a day for 3 days. The brains were removed; four from each group was processed for antioxidant assay while one from each group was fixed in Bouin’s fluid for histological studies. Our results show that chloroform inhalation adversely affected the results of the antioxidant parameters studied in a dose-dependent fashion. That means that the adverse effect worsened as the number of days increased. This was also the case with the histology results as there was evidence of cell necrosis in the cerebellar and hippocampal tissues. This also showed dose dependence. We therefore conclude from our results that when studying the brain tissues or carrying out brain related researches, chloroform inhalation is not the method of choice for rat euthanasia.
References (17)
- 1 DETERMINATION OF SERUM PROTEINS BY MEANS OF THE BIURET REACTION [DOI]
- 2 The Role of Superoxide Anion in the Autoxidation of Epinephrine and a Simple Assay for Superoxide Dismutase [DOI]
- 3 Environmental Mercury and Its Toxic Effects [DOI]
- 4 Research Methods and Design in Sport Management [DOI]
- 5 Heavy Metal Toxicity, Part I: Arsenic and Mercury [DOI]
- 6 Herophilus: The Art of Medicine in Early Alexandria: Edition, Translation and Essays
- 7 Assessing Cervical Dislocation as a Humane Euthanasia Method in Mice [DOI]
- 8 Animal Revolution: Changing Attitudes Towards Speciesism [DOI]
- 9 The Human Brain Stem and Cerebellum: Surface, Structure, Vascularization, and Three-Dimensional Sectional Anatomy, with MRI
- 10 DNA Banks for Endangered Animal Species [DOI]
- 11 Euthanasia of laboratory mice: Are isoflurane and sevoflurane real alternatives to carbon dioxide? [DOI]
- 12 Animal experimentation: The legacy of Claude Bernard [DOI]
- 13 Dietary intake of inorganic mercury: bioaccumulation and oxidative stress parameters in the neotropical fish Hoplias malabaricus [DOI]
- 14 Relationship between viral dose and outcome of infection in Atlantic salmon, Salmo salar L., post-smolts bath-challenged with salmonid alphavirus subtype 3 [DOI]
- 15 IARC Monographs on the Evaluation of Carcinogenic Risks to Humans [DOI]
- 16 The science of sacrifice: American literature and modern social theory [DOI]
- 17 Vivisection or science? An Investigation into Testing Drugs and Safeguarding Health [DOI]
Cited by 12
Ugochukwu Samuel Aguwa, Ibeh Chidubem Temple, Eze Echezona, Chioma Nwosu, David Izuchukwu Okonkwo, Adline Uchechi Uzoahia, Janeth, Adedokun, Ifeanyichukwu Michael Owoh · Journal of Complementary and Alternative Medical Research · 2022
Kamlesh Kumar Pandey, Kamakshi Mehta, Balpreet Kaur · 2023
Farida Hanum, Mona F. A. Dawood, Yoichi Atsuta · Discover Sustainability · 2024
Sabrina Salsabilah, Mustika Sari, Muthmainnah J. Abdullah · JOURNAL of HEALTH SCIENCE REVIEW · 2024
Kamlesh Kumar Pandey, Kamakshi Mehta, Balpreet Kaur · Psychopharmacology · 2024
Showing 5 of 12 known citations — external sources report more than can currently be individually listed.
Related research
- A Review - Biology, Aquaculture and Medical Use of Seahorse, Hippocampus spp — shares topic coverage
- Histological and Histochemical Study of Radiofrequency Radiation effects on the Hippocampus during the Pre- and Postnatal Stages of Development — shares topic coverage
- Positive Anti-Cadmium Poisoning Anti-Oxidant Effects of Moringa Seed Oil and Cashew Nut Oil in Juvenile Wistar Rats Hippocampus — shares topic coverage
- Histological and Histochemical Evaluation of Anticadmium Toxicity Effects of Moringa oleifera Seed Oil and Anacardium occidentale Nut Oil in the Hippocampus of Juvenile Male Wistar Rats — shares topic coverage
- In vivo Evidence of Mice Hippocampal Alterations Resulting from High Dose Caffeine Exposure during Intrauterine Life — shares topic coverage
Article metrics
Real usage data collected on this platform.
69
Page views
0
PDF downloads
33
Outbound clicks
12
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.