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Research Article Open access CC BY 4.0

Establishment of Callus Derived from Jatropha curcas L. Petiole Explants and Phytochemical Screening

K. Nyembo, N. Mbaya, N. Muambi, C. Ilunga

Biotechnology Journal International · pp. 113–118 · Published 16 Feb 2015

10.9734/BBJ/2015/15124

Abstract

Aims: The focus of this study was to assess the production of secondary metabolites of callus derived from Jatropha curcas L. petiole explants. Study Design: Laboratory experimental tests; Tissue culture, Lyophilisation, Phytochemical Analysis, Determination of tannins by Folin-Denis method. Place and Duration of the Study: Department of Phytobiology, Department of Biotechnology. General Atomic Energy Commission. Regional Center of Nuclear Studies of Kinshasa P.O BOX 868 Kin.XI DR Congo during March and June 2013. Methodology: In vitro callus cultures were initiated from petiole explants of Jatropha curcas L. on Murashige & Skoog (1962) basal medium supplemented with growth regulator formulations 4.44 μM of 6-benzylaminopurine (BAP), 4.92 μM of Indole-3-butyric acid (IBA), and 100 ml L-1 coconut milk. Callus was lyophilized. The extracts were subjected to phytochemical tests for the presence of plant secondary metabolites. The amount of tannins was estimated by Folin-Denis method. Results: Excellent growth of callus was obtained. Callus was soft, friable, lush green in color and grew fast from 8 to 30 days of culture then stabilized at low growth rate. The results obtained by phyto-chemical tests revealed the presence of saponins, tannins, alkaloids, steroids and flavonoids. The tannin contents in vitro proliferated callus, leaves, stem barks, root barks, roots and latex of J. curcas were found to be 161, 173, 220, 214, 43 and 245 μg tannic acid equivalent/g of dry weight respectively. Conclusion: The results of this study have revealed that in vitro induced callus from J. curcas petiole explants was able to produce secondary metabolites. The pharmacological activity of J. curcas reported by various researchers can be attributed to the presence of these phytochemicals.

Callus in vitro culture phytochemical tannin secondary metabolites

References (28)

  1. 1 A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures [DOI]
  2. 2 Free Radicals in the Physiological Control of Cell Function [DOI]
  3. 3 Polyphenols: food sources and bioavailability [DOI]
  4. 4 Phytochemical constituents of some Nigerian medicinal plants [DOI]
  5. 5 Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid) [DOI]
  6. 6 Medicinal Plants of East and Southeast Asia: Attributed Properties and Uses
  7. 7 Medicinal Plants of East and Southeast Asia: Attributed Properties and Uses. [DOI]
  8. 8 Antimicrobial activity and phytochemical screening of stem bark extracts from Jatropha curcas (Linn) [DOI]
  9. 9 In vitro free radical scavenging activity of five Salvia species.
  10. 10 Antifungal activities of ethanolic extract from Jatropha curcas seed cake [DOI]
  11. 11 Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon [DOI]
  12. 12 Screening of Higher Plants for Biological Activities I. Antimicrobial Activity [DOI]
  13. 13 POTENTIALLY UNLIMITED GROWTH OF EXCISED PLANT CALLUS IN AN ARTIFICIAL NUTRIENT [DOI]
  14. 14 Biodiesel production fromJatropha curcas: a critical review [DOI]
  15. 15 Potentially Unlimited Growth of Excised Plant Callus in an Artificial Nutrient [DOI]
  16. 16 Polyphenolic Contents and Antioxidant Potential of Stem Bark Extracts from Jatropha curcas (Linn) [DOI]
  17. 17 Thidiazuron (TDZ) induced plant regeneration from cotyledonary petiole explants of elite genotypes of Jatropha curcas: A candidate biodiesel plant [DOI]
  18. 18 ESTABLISHMENT OF CALLUS AND SUSPENSION CULTURE IN JATROPHA CURCAS
  19. 19 In vitro Antibacterial Activity and Phytochemical Screening of Crude Extracts from Jatropha curcas Linn. [DOI]
  20. 20 Effects of heating and the addition of seasonings on the anti-mutagenic and anti-oxidative activities of polyphenols [DOI]
  21. 21 Antimicrobial activity in leaf, seed extract and seed oil of Jatropha curcas L. plant [DOI]
  22. 22 Micropropagation of Jatropha curcas superior genotypes and evaluation of clonal fidelity by target region amplification polymorphism (TRAP) molecular marker and flow cytometry [DOI]
  23. 23 Ecofriendly Finishing of Fabric with Jatropha Curcas Leaves
  24. 24 An efficient protocol devised for rapid callus induction from leaf explants of Biophytum sensitivum (l)DC. [DOI]
  25. 25 A comparative study on the antimicrobial activity and the presence of phytochemicals in the petioles and callus of J. curcas.
  26. 26 Efficient in vitro regeneration of biodiesel plant Jatropha curcas .L
  27. 27 Amélioration du taux de multiplication in vitro de Jatropha curcas L.
  28. 28 [Review in the studies on tannins activity of cancer prevention and anticancer].

Cited by 3

Utilizing RAPD Analysis as Molecular Markers for comparison between Various Callus and Mother Plant of Jatropha curcas L.

M. Soliman, Khallil Elhalafawi, I. Ibrahim · Research Journal of Applied Biotechnology · 2018

Biotechnological approaches for the genetic improvement of Jatropha curcas L.: A biodiesel plant

Nitish Kumar, Amritpal S. Singh, S. Kumari · 2015

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