Identification of Some Acyclic Phosphate Resistant Genes in Hepatitis B Positive Subjects Attending Rivers State University Teaching Hospital

Abstract

Aim: To identify some acyclic phosphate resistant genes in hepatitis B positive subjects attending Rivers State University Teaching Hospital Study Design: Analytical Cross-sectional study. Place and Duration of Study: Rivers State University Teaching Hospital, between February and August 2024. Methodology: One hundred and thirty-eight (138) subjects aged 35 – 70 years were recruited for this study. This is made up of 44 HBV positive naïve subjects, 44 HBV Positive subjects on antiretroviral therapy (ART) and 50 subjects who are HBV negative as control subjects. DNA from the sera was extracted using quick– DNA mini-Kit supplied by Inqaba West Africa following the Zymo Research instructions. Extracted genomic DNA was quantified using the Nanodrop 100 Spectrophotometer. The S gene of the virus was amplified using nested PCR approach with primer with on a ABI 9700 applied Biosystem thermal Cycler at a final volume of 30 microlitres for 35 cycles. The data were analyzed using GraphPad Prism version 9.02. Results: The result of the Hepatitis B Resistant Genes in Subjects with Hepatitis B Infection showed that A202C was detected in 7 subjects, while T184G was detected in 10 subjects and A181T detected in 27 subjects. Conclusion: This study has shown the presence of A202C, T184G and A181T genes in some Hepatitis B positive subjects on acyclic phosphonate drug.