Skip to content
Research Article Open access CC BY 4.0

Effect of Culture Medium, Growth Regulators and Organic Additives on Shoot Regeneration from Hybrid-Seeds of Phalaenopsis

Nguyen Thi Ngoc Tram, Bui Thanh Hoa, Pham Hong Diep, Tran Van Minh

Biotechnology Journal International · pp. 45–60 · Published 5 Jun 2024

10.9734/bji/2024/v28i3723

Abstract

Aim: The Phalaenopsis Orchid, also known as the Moth Orchid, is a magnificent multicolored big orchid that has become a popular indoor plant and is easily recognized nowadays. The study of identifying the effects of culture medium, growth regulators, and organic additives on shoot growth from regenerated hybrid-seeds of Phalaenopsis is required in the first three months. Methodology: Shoots from hybrid-seeds of Phalaenopsis germination after 2 months were used as materials. Experiments were designed with the effects of nutrition mediums (MS and VW), BAP, and organic additives in Randomized Complete Block Design (CRD) via evaluation parameters on shoot growth (via number of leaves) and root growth (via root number and root length). Results: In Experiment 1, after 12 weeks, the number of leaves per shoot on 1/2xMS medium increased from 3.45 to 4.50; and root growth performed better in 1/2xMS medium (4.06 cm). In experiment 2, 1/2xMS medium + 0.05 mg/L BAP showed the highest number of roots per shoot (4.80 roots per shoot). 1⁄2xMS medium and VW medium without BAP showed the longest roots length (4.06 cm and 4.18 cm, respectively). In experiment 3, VW medium + potato showed the highest number of leaves per shoot (8.95 leaves per shoot) and VW medium + coconut water (CW) showed the longest roots length (4.78 ± 0.09 cm). in experiment 4, root growth performed better in medium enriched with CW (4.80 roots per shoot and root length 2.69 cm). Conclusion:  Both 1/2xMS and VW medium are necessary for shoot from hybrid-seeds growth in the first 6-weeks and VW medium is favored for development of shoot and root after 6-weeks.

Phalaenopsis growth regulator organic additives coconut water potato banana peptone

References (18)

  1. 1 A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures [DOI]
  2. 2 Biotechnology in agriculture and forestry
  3. 3 The Chemical Composition and Biological Properties of Coconut (Cocos nucifera L.) Water [DOI]
  4. 4 Micropropagation of orchids: A review on the potential of different explants [DOI]
  5. 5 A review for the breeding of orchids: Current achievements and prospects [DOI]
  6. 6 In vitro seed culture and seedling development of Calopogon tuberosus [DOI]
  7. 7 Rapid propagation of Phalaenopsis from floral stalk-derived leaves [DOI]
  8. 8 In Vitro Propagation and Reintroduction of the Endangered Renanthera imschootiana Rolfe [DOI]
  9. 9 The Correspondence of Charles Darwin 1878 [DOI]
  10. 10 Coconut Water and Peptone Improve Seed Germination and Protocorm Like Body Formation of Hybrid Phalaenopsis
  11. 11 Improvement of asymbiotic seed germination and seedling development ofCypripedium macranthosSw. with organic additives [DOI]
  12. 12 Commercial fertilizers and organic additives in orchid micropropagation [DOI]
  13. 13 Evaluation of basal media for micropropagation of four highbush blueberry cultivars [DOI]
  14. 14 Clonal multiplication of orchids
  15. 15 A Study on the Use of Organic Additives on the Protocorm-like Bodies (PLBS) Growth of Phalaenopsis violacea Orchid
  16. 16 Photosynthetic Light Requirements and Effects of Low Irradiance and Daylength on Phalaenopsis amabilis [DOI]
  17. 17 Peptone stimulates in vitro shoot and root regeneration of avocado (Persea americana Mill.) [DOI]
  18. 18 EFFECTS OF TOTAL NITROGEN AND BA ON IN VITRO CULTURE OF PHALAENOPSIS [DOI]

Cited by 6

The Effect of Different Media and BAP in Planlet Growth of Phalaenopsis OX Red Lion ‘The Conqueror’

Sri Hartati, Rafidah Agnesa Candra Kirana · IOP Conference Series: Earth and Environmental Science · 2025

Enhancing cut Phalaenopsis preservation by the combination of Slightly Acidic Electrolytic Water-based Preservative Solution (SAEW-PS) with Low Voltage Electrostatic Field (LVEF)

Jiamiao Hu, Lifang Liu, Chang-Jer Wu · Scientia Horticulturae · 2025

Showing 2 of 6 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

8

Page views

0

PDF downloads

0

Outbound clicks

6

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.