Skip to content
Research Article Open access CC BY 4.0

Effect of Cultural Conditions on Biosurfactant Production by Candida sp. Isolated from the Sap of Elaeis guineensis

I. V. Nwaguma, C. B. Chikere, G. C. Okpokwasili

Biotechnology Journal International · pp. 1–14 · Published 8 Aug 2019

10.9734/bji/2019/v23i330079

Abstract

Aims: This study is aimed at determining the effect of cultural condition on biosurfactant production by Candida sp. isolates from saps of Elaeis guineensis. Methodology: Chemical analysis of the sap was carried out. Yeast isolates from the sap were screened for biosurfactant production based on emulsification index (E24), emulsification assay, haemolytic assay, oil displacement test, CTAB and tilted glass slide ability. The best biosurfactant-producing yeast isolate was identified based on its phenotypic, microscopic, and biochemical characteristics. The emulsification capacity of the produced biosurfactant on selected oils was studied. Optimum cultural and nutritional requirements (temperature, pH, inoculum       concentration, nitrogen sources and carbon sources) for biosurfactant production by the isolate were determined. Results: The characteristics of the sap from Elaeis guineensis were reducing sugar (0.51 ± 0.03 mg/ml), alcohol (14.04 ± 0.15%), specific gravity (0.827±0.024), and pH (5.68±0.03). The crude biosurfactant produced displaced a thin film of crude oil on petri dish by 55 mm, and revealed high emulsification index (E24) of 52.5% using Olive oil as substrate compared to E24 of 60.6% by sodium dodecyl sulphate (SDS). Based on colonial, microscopic, and biochemical characteristics, the isolate SA2 was identified as Candida sp. The crude biosurfactant showed varying capacity in emulsifying the different oils that were examined. Optimization data revealed maximum biosurfactant production after 7 days of incubation, inoculum concentration of 10%, at temperature of 20ºC, pH of 2 with cassava peel as substrate. Conclusion: The study has demonstrated the capacity of Candida sp. from the sap of Elaeis guineensis to produce biosurfactant utilizing cassava peel as substrate. The use of cassava peel, which represents a low-cost substrate, is important in reducing the cost of biosurfactant production. Moreover, using yeasts from Elaeis guineensis make the production process ecologically friendly.

Biosurfactant Candida sp optimization Elaeis guineensis

References (41)

  1. 1 Biosurfactants: Multifunctional Biomolecules of the 21st Century [DOI]
  2. 2 Comparison of methods to detect biosurfactant production by diverse microorganisms [DOI]
  3. 3 Microbial biosurfactants: challenges and opportunities for future exploitation [DOI]
  4. 4 Biological amphiphiles (microbial biosurfactants) [DOI]
  5. 5 Isolation and selection of biosurfactant-producing bacteria [DOI]
  6. 6 Screening and production of rhamnolipids by Pseudomonas aeruginosa 47T2 NCIB 40044 from waste frying oils [DOI]
  7. 7 Glycerol as substrate for the production of biosurfactant by Pseudomonas aeruginosa UCP0992 [DOI]
  8. 8 Microbial Surfactants: A Review [DOI]
  9. 9 Assessment of different screening methods for selecting biosurfactant producing marine bacteria
  10. 10 Production and stability studies of the biosurfactant isolated from marine Nocardiopsis sp. B4 [DOI]
  11. 11 Biological activity of glycolipids produced by microorganisms: New trends and possible therapeutic alternatives [DOI]
  12. 12 A Review on Biosurfactants: Fermentation, Current Developments and Perspectives
  13. 13 Sophorolipid biosynthesis by Candida bombicola from industrial fatty acid residues [DOI]
  14. 14 The effect of medium composition on the structure and physical state of sophorolipids produced by Candida bombicola ATCC 22214 [DOI]
  15. 15 Enhancement of stability of biosurfactant produced by Candida lipolytica using industrial residue as substrate [DOI]
  16. 16 Biosurfactant-producing bacterium, Pseudomonas aeruginosa MA01 isolated from spoiled apples: Physicochemical and structural characteristics of isolated biosurfactant [DOI]
  17. 17 Biotechnological opportunities in biosurfactant production [DOI]
  18. 18 Isolation, characterization, and application of biosurfactant by Klebsiella pneumoniae strain IVN51 isolated from hydrocarbon-polluted soil in Ogoniland, Nigeria [DOI]
  19. 19 The isolation of a thermophilic biosurfactant producing Bacillus SP [DOI]
  20. 20 Evaluation of sophorolipid biosurfactant production by Candida bombicola using animal fat [DOI]
  21. 21 Microbial conversion of n-alkanes into glycolipid biosurfactants, mannosylerythritol lipids, by Pseudozyma (Candida antarctica) [DOI]
  22. 22 Application of oil refinery waste in the biosynthesis of glycolipids by yeast [DOI]
  23. 23 Selection of microorganisms for biosurfactant production using agroindustrial wastes [DOI]
  24. 24 Production of sophorolipids by Candida bombicola grown on soy molasses as substrate*,** [DOI]
  25. 25 Production and stability studies of the bioemulsifier obtained from a new strain of Candida glabrata UCP 1002 [DOI]
  26. 26 A new biosurfactant produced by Candida glabrata UCP 1002: characteristics of stability and application in oil recovery [DOI]
  27. 27 Capacity for biosurfactant production of environmental Pseudomonas and Vibrionaceae growing on carbohydrates [DOI]
  28. 28 Draft Genome Sequence of the Basidiomycetous Yeast-Like Fungus Pseudozyma hubeiensis SY62, Which Produces an Abundant Amount of the Biosurfactant Mannosylerythritol Lipids [DOI]
  29. 29 CHARACTERIZATION OF SOPHOROLIPID BIOSURFACTANT PRODUCED BY YEAST SPECIES GROWN ON DIESEL OIL
  30. 30 Distribution and in vitro antimicrobial susceptibility of Acinetobacter species on the skin of healthy humans.
  31. 31 Production of glycolipids containing biosurfactant by Pseudomonas species.
  32. 32 Economic Optimized Medium for Tensio-Active Agent Production by Candida sphaerica UCP0995 and Application in the Removal of Hydrophobic Contaminant from Sand [DOI]
  33. 33 Isolation and Characterization of Palm Wine Strains of Saccharomyces cerevisiae Potentially Useful as Bakery Yeasts [DOI]
  34. 34 Petroleum Hydrocarbon Spills in the Environment and Abundance of Microbial Community Capable of Biosurfactant Production [DOI]
  35. 35 A study on bacterial and fungal diversity in potted soil.
  36. 36 Biosurfactant stabilized anticancer biomolecule-loaded poly (d,l-lactide) nanoparticles [DOI]
  37. 37 Bioethanol Production by an Ethanol-Tolerant Bacillus cereus Strain GBPS9 Using Sugarcane Bagasse and Cassava Peels as Feedstocks [DOI]
  38. 38 Properties of palm wine yeasts and its performance in wine making
  39. 39 Biosurfactants Produced by Genetically Manipulated Microorganisms: Challenges and Opportunities [DOI]
  40. 40 Characterization of biosurfactant produced from submerged fermentation of fruits bagasse of yellow cashew (Anacardium occidentale) using Pseudomonas aeruginosa [DOI]
  41. 41 Screening, Optimization and Production of Biosurfactants by Candida Species Isolated from Oil Polluted Soils

Cited by 12

Microbial Biosurfactants in Food Processing Industry

Muhammad Bilal Sadiq, Muhammad RehanKhan, R. Z. Sayyed · Multifunctional Microbial Biosurfactants · 2023

Biosurfactants: Production and application prospects in the food industry

Beatriz G. Ribeiro, Jenyffer M. C. Guerra, Leonie A. Sarubbo · Biotechnology Progress · 2020

Biosurfactants Produced by Yeasts: Fermentation, Screening, Recovery, Purification, Characterization, and Applications

Natalia de Andrade Teixeira Fernandes, Luara Aparecida Simões, Disney Ribeiro Dias · Fermentation · 2023

Biofuels: Bioethanol, biogas, and biohydrogen production from cassava peels

Ferdinand Tornyie, Kofi Ampomah-Benefo, Mawuli Dogli · Sustainable Cassava · 2024

Natural Antifungal Agents Isolated from Argentine Plants. A Summary of Studies Developed in the Period 2000–2020

Gisela Seimandi, Estefanía Butassi, Melina Di Liberto · Promising Antimicrobials from Natural Products · 2022

Isolation, Characterization and Identification of Yeast in Pindang Eggs as a Starter for Fermented Chicken Eggs

Andry Pratama, Jajang Gumilar, Wendry S. Putranto · Asian Journal of Dairy and Food Research · 2026

Showing 6 of 12 known citations — external sources report more than can currently be individually listed.

Article metrics

Real usage data collected on this platform.

56

Page views

0

PDF downloads

17

Outbound clicks

12

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.