Skip to content
Research Article Open access CC BY 4.0

Distribution and Antibiotic Resistance Profile of Extended Spectrum Beta-Lactamase Producing Escherichia coli from Fish Farms within Abakaliki Metropolis

Jennifer Onyekachi Adibe-Nwafor, Uduku , Nwadaonwe Demian, Nwadaonwe Demian Uduku, Iroha , Chidinma Stacy, Chidinma Stacy Iroha, Onuora , Adaora Lynda, Francis Amadi Ibiam, Adaora Lynda Onuora, Peter , Ikemesit Udeme, Kenneth Adibe Nwafor, Ikemesit Udeme Peter, Ifeanyichukwu Romanus Iroha, Ibiam , Francis Amadi, Iroha , Ifeanyichukwu Romanus, Nwafor , Kenneth Adibe

Advances in Research · pp. 175–184 · Published 21 Jul 2023

10.9734/air/2023/v24i5968

Abstract

Background and Objectives: The emergence of antibiotic resistant determinant in fish farms and its spread is on the increase and has evolved into strains that are resistant to many classes of antibiotics. Thus, it is critical to identify the distribution and antibiotic resistance profile of Extended Spectrum Beta-lactamase (ESBL) producing Escherichia coli from fish farms within Abakaliki Metropolis. Methodology: Aseptically, fifty (50) milliliters of fishpond water was collected from twenty locations in fifteen (15) fish farms and were analyze using standard microbiological culture and identification of Escherichia coli. Detection of phenotypic extended spectrum β-lactamases production was performed using Double-Disk Synergy Test (DDST). Antibiotic susceptibility studies of extended spectrum β-lactamases producing Escherichia coli was determined using the Kirby–Bauer disk diffusion method and the results were construed using the Clinical Laboratory Standard Institute (CLSI) zone diameter breakpoints. Results: Extended spectrum beta-lactamase producing Escherichia coli distribution from fishpond water revealed overall occurrence rate of 34(11.3 %). The proportion of ESBL producing Escherichia coli was 5(25.0 %) from fish farm L followed by Farm A, Farm E, Farm G, which both accounted for 20.0 % respectively while the least occurrence of 1(5.0 %) was recorded against Farm I. ESBL-producing E. coli were resistant to cephalosporin particularly Ceftriaxone (88.2%), Ceftazidime (91.2 %), Cefotaxime (94.1) and Cefepime (85.3 %). This was followed by Amoxicillin-Clavulanate (91. 2 %), Azetronam (97.1 %). In all, Ciprofloxacin (82.4 %), Imipenem (97.1 %), and Meropenem (100 %) were the most effective antibiotic against ESBL-producing E. coli isolate. Conclusion: This study reveals the prevalence of the ESBL phenotype in fish farms. The increasing prevalence of resistance to routinely used antibiotics in medical and veterinary therapies among the study isolates from aquaculture products poses a significant challenge to the treatment of human and animal diseases. As a result, adequate antibiotic intervention is essential to ensure the continued efficacy of antibiotics for aquaculture and human health, as well as the industry's viability.

Escherichia coli extended spectrum beta-lactamase antibiotic resistance fish farm

References (31)

  1. 1 The Rising Tide of Antimicrobial Resistance in Aquaculture: Sources, Sinks and Solutions [DOI]
  2. 2 Evaluating antimicrobial resistance in the global shrimp industry [DOI]
  3. 3 Twenty-year trends in antimicrobial resistance from aquaculture and fisheries in Asia [DOI]
  4. 4 Overview of aquaculture systems in Egypt and Nigeria, prospects, potentials, and constraints [DOI]
  5. 5 Detection and occurrence of indicator organisms and pathogens [DOI]
  6. 6 Antimicrobial Profiling of Bacteria Isolated from Fish Sold at Informal Market in Mufakose, Zimbabwe [DOI]
  7. 7 Antibiotic Resistance Profiles and Molecular Characteristics of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli and Klebsiella pneumoniae Isolated From Shrimp Aquaculture Farms in Kerala, India [DOI]
  8. 8 Prevalence of common carbapenemase genes and multidrug resistance among uropathogenic Escherichia coli phylogroup B2 isolates from outpatients in Wasit Province/ Iraq [DOI]
  9. 9 Predominance of CTX-M-15 among ESBL Producers from Environment and Fish Gut from the Shores of Lake Victoria in Mwanza, Tanzania [DOI]
  10. 10 Extended-Spectrum Beta-Lactamase Producing-Escherichia coli Isolated From Irrigation Waters and Produce in Ecuador [DOI]
  11. 11 The Impact of Intensive Fish Farming on Pond Sediment Microbiome and Antibiotic Resistance Gene Composition [DOI]
  12. 12 Antimicrobials and resistant bacteria in global fish farming and the possible risk for public health [DOI]
  13. 13 Plasmid-Mediated Antibiotic Resistant Escherichia coli in Sarawak Rivers and Aquaculture Farms, Northwest of Borneo [DOI]
  14. 14 Prevalence of Enterobacteriaceae on fresh produce and food safety practices in small-acreage farms in Tennessee, USA [DOI]
  15. 15 Geotechnical and environmental problems related to shales in the Abakaliki area, Southeastern Nigeria [DOI]
  16. 16 Prevalence and antibiotic susceptibility pattern of Escherichia coli O157:H7 isolated from harvested fish at Lake Hayq and Tekeze dam, Northern Ethiopia [DOI]
  17. 17 Resistance Determinants of Pseudomonas Species from Aquaculture in Australia [DOI]
  18. 18 A review of fresh produce outbreaks, current interventions, food safety concerns and potential benefits of novel antimicrobial sodium acid sulfate [DOI]
  19. 19 Antibiotic sensitivity patterns of microbial isolates from fish ponds: A study in the Greater Accra Region of Ghana [DOI]
  20. 20 Bacteriological profile of selected fish species and water sample from Otuocha River Anambra State [DOI]
  21. 21 Comparison of Antibiotic-Resistant Pattern of Extended Spectrum Beta-Lactamase and Carbapenem-Resistant Escherichia Coli Isolates from Clinical and Non-Clinical Sources [DOI]
  22. 22 Surveillance of Escherichia coli in a fish farm of Sylhet, Bangladesh [DOI]
  23. 23 Detection of Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae Implicated in Urinary Tract Infection [DOI]
  24. 24 Killing Rate Kinetics of Commercially Available Brands of Ciprofloxacin and Cefotaxime on Clinical Bacterial Isolates Subjected to in vitro Antibiotic Treatments [DOI]
  25. 25 Prevalence and Antibiogram Profile of Carbapenem-resistant Escherichia coli and Klebsiella pneumoniae among Patients with Urinary Tract Infection in Abakaliki, Nigeria [DOI]
  26. 26 Antibiotic resistance and molecular characteristics of extended-spectrum beta-lactamase-producing Escherichia coli isolated from fish pond [DOI]
  27. 27 Antibiotic Susceptibility Profile of Clinical Isolate of Carbapenem-Resistant Pseudomonas aeruginosa [DOI]
  28. 28 Phenotypic Detection of AmpC \(\beta\)-lactamase Producing Escherichia coli among Patients in Hospital Wards [DOI]
  29. 29 Phenotypic screening of multidrug-resistant E. coli from water and fish collected from different fish farms within Abakaliki metropolis, Nigeria [DOI]
  30. 30 Pisciculture and Fish Parts as Non-clinical Source of CTX-M and TEM Extended Spectrum Beta-lactamases Producing Escherichia coli in Southeastern Nigeria [DOI]
  31. 31 Prevalence of extended spectrum beta lactamase producing escherichia coli in integrated agro-aquaculture in Morogoro, Tanzania

Cited by 9

Antibiotic resistance in Nigeria’s water system: an urgent public health challenge impeding the achievement of Sustainable Development Goals (SDGs)

Emmanuel Sunday Okeke, Johnbosco C Egbueri, Stephen Sunday Emmanuel · Integrated Environmental Assessment and Management · 2025

Antimicrobial resistance of Escherichia coli isolated from fish and aquaculture water: an emerging concern for consumers

Luan Amaral Alexandre, Alice Cristina da Silva, Francisco Lucas de Amorim Nascimento · Letters in Applied Microbiology · 2025

Characterization of VIM, VEB and CTX-M Beta-lactamase Gene in Escherichia coli and Pseudomonas aeruginosa Isolated from Urine Samples of Patients Visiting a Tertiary Hospital in Abakaliki

Donatus Chukwuma Ilang, Ikemesit Udeme Peter, Ifeanyichukwu Romanus Iroha · International Journal of Medical Sciences and Pharma Research · 2023

Showing 3 of 9 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

71

Page views

0

PDF downloads

43

Outbound clicks

9

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.