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Research Article Open access CC BY 4.0

Pisciculture and Fish Parts as Non-clinical Source of CTX-M and TEM Extended Spectrum Beta-lactamases Producing Escherichia coli in Southeastern Nigeria

Jennifer Onyekachi Adibe-Nwafor, Iroha , Chidinma Stacy, Ikemesit Udeme Peter, Peter , Ikemesit Udeme, Kenneth Adibe Nwafor, Chidinma Stacy Iroha, Adaora Lynda Onuora, Edemekong , Christiana Inuaesiet, Christiana Inuaesiet Edemekong, Francis Amadi Ibiam, Ifeanyichukwu Romanus Iroha, Onuora, Adaora Lynda, Ibiam , Francis Amadi, Iroha , Ifeanyichukwu Romanus

Asian Journal of Fisheries and Aquatic Research · pp. 18–25 · Published 13 Jul 2023

10.9734/ajfar/2023/v23i5613

Abstract

Background and Objectives: Antimicrobial agent use in pisciculture exerts a selective pressure, resulting in a reservoirs of extended spectrum beta-lactamase producing Escherichia coli in the aquatic environment.  Here we screened pisciculture and fish parts as non-clinical source of CTX-M and TEM Extended Spectrum Beta-lactamases (ESBL) producing Escherichia coli in Southeastern Nigeria Methodology: A total of sixty fish parts [fish gills, body part and intestinal swab samples] were collected from pisciculture farm A, C, F and G within Abakaliki metropolis. The samples were bacteriologically analyzed using standard microbiological techniques for isolation and identification. ESBL enzymes were phenotypically detected in E. coli, isolates using the double disk diffusion technique. The presence of TEM and CTX-M genes was determined by polymerase chain reaction using specific primers. Results: Escherichia coli and Extended spectrum beta-lactamase producing Escherichia coli accounted for overall occurrence rate of 27(45.0 %) and 17(28.3 %) respectively. There was no statistically significant difference in the occurrence of Escherichia coli and ESBL-E. coli among the different samples P>0.05. The presence of TEM and CTX-M gene 80 % and 100 % respectively were confirmed among the isolates. Conclusion: Our findings reports the presence of ESBL gene in pisciculture and fish parts and it is important in understanding the mechanism of resistance operating in these common pathogens, which are also endemic in most pisciculture area. Strict rules and monitoring/surveillance of antimicrobial agent used in pisciculture activities combined with food safety training of farmer owners/breeders on various aspects of good hygiene practices are strongly recommended.

Pisciculture CTX-M TEM Escherichia coli

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