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Different Morphotypes of Fusarium oxysporum Isolated from Xanthosoma sagittifolium L. Schott Roots: Action of Ethanol Leaf Extracts of Psidium guajava on their in Vitro Inhibition and on X. Sagittifolium Plants Inoculated with F. oxysporum

A. C. Djeuani, H. D. Mbouobda, T. A. Mbekem, N. Niemenak

International Journal of Pathogen Research · pp. 25–41 · Published 27 Nov 2020

10.9734/ijpr/2020/v5i430142

Abstract

The objective of this study was to determine the different morphotypes of Fusarium oxysporum present in the root of Xanthosoma sagittifolium and evaluate the effect of alcoholic extracts of Psidium guajava on their in vitro inhibition. Strains of Fusarium oxysporum were collected in eight localities where X. sagittifolium is grown. Fusarium strains isolated from roots of X. sagittifolium harvested in each locality were grown on PDA medium. The antifungal test was evaluated using ethanol extracts from P. guajava leaves at 30 and 60%. The virulence test of each strain on young plants of X. sagittifolium aged three months were realized. Eight strain of Fusarium oxysporum were successfully isolated. After maximum growth, five morphological types were observed (pionnotal, sclerotic, clowny, cottony and ras senescent). The cottony strain was abundant and present in all the locality. Histological analysis of the different strains obtained revealed the presence of septate or siphoned hyphae and three types of conidia (microconidia, macroconidia and sporangiospores or chlamidospores). The inhibition tests were very high with 60% of ethanol extract of P. guajava, and 83.33% of inhibitory effect were observed after eight days of growth, in the strains collected in X. sagittifolium roots, in L3 (Loum) and L4 (Bangoua) localities. After infection of X. sagittifolium plants with each strain of F. oxysporum isolated, symptoms observed were yellowing and wilting of leaves. However, plants inoculated with the L3 (Loum) strain showed both yellowing and wilting of leaves. The application of ethanol extracts from P. guajava leaves reduced the severity of the disease in the inoculated plants after 14 days. These results obtained showed that F. oxysporum is not only saprophytic fungi, it’s also able to induce yellowing and wilting of leaves in X. sagittifolium.

Fusarium oxysporum Xanthosoma sagittifolium L Schott Psidium guajava inhibition antifungal test.

References (10)

  1. 1 Fusarium species: an illustrated manual for identification.
  2. 2 Techniques for evaluation of medicinal plant products as antimicrobial agents: Current methods and future trends [DOI]
  3. 3 The presence of a virulence locus discriminates Fusarium oxysporum isolates causing tomato wilt from other isolates [DOI]
  4. 4 Endophytic fungi from Musa acuminata leaves and roots in South China [DOI]
  5. 5 Effect of Some Natural Extracts on Growth and Chemical Constituents of Schefflera arboricola Plants
  6. 6 Triterpenoids from Psidium guajava with biocidal activity [DOI]
  7. 7 Psidium guajava extract reduces trypanosomosis associated lipid peroxidation and raises glutathione concentrations in infected animals [DOI]
  8. 8 Antifungal Activity of Selected Medicinal Plant Extract on Fusarium oxysporum Schlechtthe Causal Agent of Fusarium Wilt Disease in Tomato [DOI]
  9. 9 Characterization of Fusarium oxysporum isolates from tomato plants in Algeria [DOI]
  10. 10 Bio-suppression of Fusarium Wilt Disease in Potato Using Nonpathogenic Potato-associated Fungi [DOI]

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