Skip to content
Research Article Open access CC BY 3.0

Development of Quality Protein Maize (QPM) Inbred Lines and Genetic Diversity Assessed with ISSR Markers in a Maize Breeding Program

K. Mbuya, K. K. Nkongolo, R. Narendrula, A. Kalonji-Mbuyi, R. V. Kizungu

Journal of Experimental Agriculture International · pp. 626–640 · Published 2 Nov 2012

10.9734/AJEA/2012/1626

Abstract

Very few studies have been conducted to develop new quality protein maize (QPM) varieties adapted to various agro-ecological conditions in Africa. Such QPM varieties with high grain yield, genes for resistance to local disease and pests would contribute to increased grain production and to alleviate poverty and malnutrition. During the first step of the QPM breeding program in the DR-Congo, 137 inbred lines were developed and selected in the field based on various agro-morphometric characteristics. Molecular analysis revealed high level of genetic variability among inbreds. The level of polymorphic loci observed with ISSR markers varied between 74% and 80%. Nei’s gene diversity and Shannon’s information index values varied from 0.22 to 0.27 and from 0.34 to 0.41, respectively. The majority of inbred lines were distantly related. In fact, more than 87% of genetic distance values were above 0.50. The genetic distance values among the different parental maize accessions varied from 0.39 to 0.72. Inbred lines from the same parental accession were also not genetically close with genetic distance values varying from 0.28 to 0.59. More importantly, the ISSR data are useful in the selection of inbred lines to be tested for general and specific combining abilities and further development of synthetics.

Quality protein maize (QPM) DR-Congo inbreds ISSR genetic variability.

References (40)

  1. 1 Genome Fingerprinting by Simple Sequence Repeat (SSR)-Anchored Polymerase Chain Reaction Amplification [DOI]
  2. 2 Quantitative Genetics in Maize Breeding
  3. 3 Structure of linkage disequilibrium and phenotypic associations in the maize genome [DOI]
  4. 4 Analysis of Genetic Diversity in Crop Plants—Salient Statistical Tools and Considerations [DOI]
  5. 5 Population genetic analysis of codominant and dominant markers and quantitative traits
  6. 6 Dwarf8 polymorphisms associate with variation in flowering time [DOI]
  7. 7 Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers [DOI]
  8. 8 Genetic Structure and Diversity Among Maize Inbred Lines as Inferred From DNA Microsatellites [DOI]
  9. 9 SNP frequency, haplotype structure and linkage disequilibrium in elite maize inbred lines [DOI]
  10. 10 Wheat genetic diversity trends during domestication and breeding [DOI]
  11. 11 Potential of Molecular Markers in Plant Biotechnology
  12. 12 Molecular characterization of global maize breeding germplasm based on genome-wide single nucleotide polymorphisms [DOI]
  13. 13 Genetic Distance Based on Simple Sequence Repeats and Heterosis in Tropical Maize Populations [DOI]
  14. 14 Breeding and disseminating quality protein maize (QPM) for Africa [DOI]
  15. 15 Molecular characterization of diverse CIMMYT maize inbred lines from eastern and southern Africa using single nucleotide polymorphic markers [DOI]
  16. 16 Use of SSRs for establishing heterotic groups in subtropical maize [DOI]
  17. 17 Gene mapping with recombinant inbreds in maize. [DOI]
  18. 18 Genetic diversity for restriction fragment length polymorphisms and heterosis for two diallel sets of maize inbreds [DOI]
  19. 19 Classification of North Dakota maize inbred lines into heterotic groups based on molecular and testcross data [DOI]
  20. 20 Combining Abilities of Quality Protein Maize Inbreds [DOI]
  21. 21 High-lysine maize: the key discoveries that have made it possible [DOI]
  22. 22 Review article: Quality protein maize (QPM): Genetic manipulation for the nutritional fortification of maize [DOI]
  23. 23 Use of ISSR fingerprints to detect microsatellites and genetic diversity in several related Brassica taxa and Arabidopsis thaliana [DOI]
  24. 24 Nutritional Analysis of Quality Protein Maize Varieties Selected for Agronomic Characteristics in a Breeding Program [DOI]
  25. 25 Genetic analysis of Pinus strobus and Pinus monticola populations from Canada using ISSR and RAPD markers: development of genome-specific SCAR markers [DOI]
  26. 26 Regulation of maize lysine metabolism and endosperm protein synthesis by opaque and floury mutations [DOI]
  27. 27 Genetic analysis of mutations that alter cell fates in maize leaves: DominantLiguleless mutations [DOI]
  28. 28 Genetic mapping in maize with hybrid progeny across testers and generations: plant height and flowering [DOI]
  29. 29 Combining ability of early-maturing quality protein maize inbred lines adapted to Eastern Africa [DOI]
  30. 30 Quality Protein Maize: A Biochemical Study of Enzymes Involved in Lysine Metabolism [DOI]
  31. 31 Inheritance of photosynthesis in a diallel among eight maize inbred lines from Iowa Stiff Stalk Synthetic [DOI]
  32. 32 Participatory selection and characterization of quality protein maize (QPM) varieties in Savanna agro- ecological region of DR-Congo [DOI]
  33. 33 Evaluation of maize S2 lines in test cross combinations. I: Flowering and morphological traits.
  34. 34 Molecular analysis of quality protein (QPM) and normal maize varieties from the DR-Congo breeding program [DOI]
  35. 35 Regulation of Lysine Metabolism and Endosperm Protein Synthesis by the Opaque-5 and Opaque-7 Maize Mutations [DOI]
  36. 36 Comparison of Selection for S2 Progeny vs. Testcross Performance for Population Improvement in Maize [DOI]
  37. 37 Early generation identification and utilization of potential inbred lines in modified single cross hybrids of maize (Zea mays L.)
  38. 38 Genetic relationships among cimmyt subtropical QPM and Chinese maize inbred lines based on SSRS
  39. 39 Evaluation of maize varieties for their potential as breeding populations [DOI]
  40. 40 Evaluation of S2 Maize Lines Reproduced for Several Generations by Random Mating within Lines. I. Comparisons between the Original and Maintained S2 Lines [DOI]

Cited by 5

Showing 2 of 5 known citations — external sources report more than can currently be individually listed.

Article metrics

Real usage data collected on this platform.

14

Page views

0

PDF downloads

0

Outbound clicks

5

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.