Detection of Androgen Receptor Gene Polymorphism in Sudanese Patients of Prostate Cancer in Khartoum State

Abstract

Background: Prostate cancer (PCa) is the most commonly diagnosed solid tumor among men. Genetic susceptibility had been proposed among the risk factors for the development of this cancer. Aim: To investigate the effect of androgen receptor gene polymorphism in the susceptibility of prostate cancer among Sudanese patients. Methods: This study was conducted in Khartoum State during the period from December 2021 to May 2022. The study population that was selected consisted of one hundred patients, who had prostate cancer, who attended for routine follow-up assessment following their chemotherapy treatment. A total of 5 ml EDTA anti-coagulated venous blood samples were obtained from all participants. Prostate specific antigen (PSA) was measured by competitive chemiluminescence immunoassay. DNA extraction was performed for all samples by chemical method and genotyping was performed by PCR-RFLP method using Eco147I enzyme. Results: The Androgen receptor genotype showed that wild (G/G) type was more frequent (89%) than heterozygous (G/A) type (11%), and allele G was more frequent (94.5%)  than allele A (5.5%). The mean serum PSA level among prostate cancer patients was 18.71+31.68 ng/ml. Comparison of the mean serum PSA levels between different AR genotypes revealed no significant association between the genotype and the hormonal level (p. values > 0.05). Conclusion: Androgen receptor gene polymorphism was detected in this study population as heterozygous type. Moreover; there was no significant association between AR genotype and serum PSA level.