Coat Protein Characterization of Mungbean yellow mosaic virus Infecting Blackgram (Vigna mungo L. Hepper) in Tamil Nadu
Nimmala Sree Valli, G. Karthikeyan, S. Harish, M. Sudha, D. Jeya Sundara Sharmila
International Journal of Plant & Soil Science · pp. 638–644 · Published 12 Aug 2022
10.9734/ijpss/2022/v34i2231418Abstract
The Yellow Mosaic Disease is the main constraint for decreased yield in blackgram production. Coat protein gene of Mungbean yellow mosaic virus (MYMV) has been characterized and phylogenetic tree was constructed. The genomic DNA from diseased plants was collected and it was tested for it’s quality and quantity using a Nanodrop. The isolated DNA was subjected to polymerase chain reaction using begomovirus universal primers for preliminary confirmation of the virus. The samples amplified for universal primers were subjected for gene specific amplification using DNA A primers. The DNA A primers resulted in the amplification of the expected size of 1000 bp amplicon. The DNA fragments were sequenced and the sequence results of DNA A were compared with other sequences available in NCBI and it showed higher similarity with Mungbean yellow mosaic virus banglore coat protein gene (accession no: MK409376). The sequence was submitted in NCBI database and accession numbers were obtained. The phylogenetic tree was constructed using mega 11 software by comparing the selected strains with our isolate.
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