Antimicrobial Efficacy of Methanolic and Aqueous Extracts of Partially Purified Protein from Young and Matured Root of Guiera senegalensis (Moshi Medicine)

Abstract

Background: Antibiotic resistance among pathogenic bacteria is increasing at an alarming rate leading to the need for traditional medicine as an alternative. Aim of the Study: The study aimed to evaluate the antimicrobial activity of methanolic and, aqueous extracts of partial purified protein of young and matured roots of Guiera senegalensis. Methodology: Antimicrobial activity was determined by disc diffusion and broth dilution techniques, Quantitative phytochemical analysis was carried out by standard procedure, the gel chromatography technique was used to fractionate the crude protein. The test isolates were Bacillus subtilis, Staphylococcus aureus, E. coli, Salmonella typhimurium and Candida albicans. Results: The antimicrobial activity showed all the extracts were quite effective against most of the test isolates except Candida albicans (fungus). The crude and partially purified proteins were active against Gram-positive bacteria. The maximum zone of inhibition (37.33±5.03b mm) was observed in methanolic extracts of young root against Staphylococcus aureus at 100 mg/ml. Most extracts of methanolic exhibited minimum inhibitory concentration (MIC) at the range of 6.25 mg/ml and 12.5 mg/ml and minimum bactericidal concentration (MBC) at 12.5 mg/ml and 25 mg/ml. The young root was more active than the matured root. Quantitative phytochemicals showed a high amount of saponins (9.98% and 6.42%) in matured and young roots respectively. Conclusion: Guiera senegalensis has broad-spectrum antimicrobial activity and a potential source of new antibiotics that could be useful for the treatment of infectious diseases.