Cefepime-resistance in Clinical Isolates of Pseudomonas aeruginosa and Klebsiella spp in Ogbomoso

Abstract

Aims: Our goal was to identify resistance rate of Cefepime-resistant Pseudomonas aeruginosa and Klebsiella spp infections in the hospital setting within Ogbomoso. Study Design: The study includes clinical isolates of Klebsiella spp and Pseudomonas aeruginosa obtained by screening samples of blood (for blood culture), urine, aspirate, wound, throat, sputum etc. Place and Duration of Study: Clinical isolates were obtained from Medical Microbiology and Parasitology Laboratory of the Ladoke Akintola University of Technology Teaching Hospital, Ogbomoso, between March and August 2013. Methodology: Sixty-six clinical isolates of Pseudomonas aeruginosa and Klebsiella spp obtained from different clinical sites were analyzed using standard bacteriological methods. The sensitivity of clinical isolates of Pseudomonas aeruginosa and Klebsiella spp to Cefepime (30µg) was determined using disk diffusion method. The isolates were divided into three groups sensitive, intermediate and resistance. Results: The results shows that Pseudomonas aeruginosa had 34.38% sensitivity, 6.25% intermediate and 59.37% resistance while Klebsiella spp had 41.18% sensitivity, 5.88% intermediate and 52.94% resistance. Overall susceptibility pattern of the clinical isolates to Cefepime is 37.87% sensitivity, 6.06% intermediate and 56.06% resistance. Conclusion: There was a great Cefepime–resistance among the clinical isolates analyzed. The resistance pattern of Pseudomonas aeruginosa and Klebsiella spp calls for continuous surveillance for Cefepime resistance control.