Antimicrobial Activity of Potato Rhizospheric Pseudomonas chlororaphis subsp. aureofaciens from Sétif Algeria
Samia Mezaache-Aichour, Nora Haichour, Jane Nicklin, Mohamed M. Zerroug
Annual Research & Review in Biology · pp. 1–7 · Published 6 Feb 2017
10.9734/ARRB/2016/31651Abstract
Aims: This study was assessed to demonstrate the antimicrobial activity in vitro of an identified fluorescent Pseudomonas strain characterized for its capacity to produce phenazine compounds. Methodology: First Pseudomonas chlororaphis subsp aureofaciens was inoculated on Nutrient Broth supplemented with Yeast Extract (NBY) and with glucose at a final concentration of 2%, after incubation the filtered culture was acidified with HCl to pH 2. The solution was extracted twice with the same volume of ethyl-acetate. The organic supernatants were combined, dried over anhydrous Na2SO4, and evaporated to dryness. The crude extract was resuspended in methanol and tested for antimicrobial activity. Antimicrobial activity was determined (i) by disc diffusion technique for bacteria and (ii) using serial dilution technique in soft PDA for fungi. Secondly the antifungal activity of the bacterial strain was tested against several phytopathogenic fungi in dual culture. Results: The studied strain has an important activity against the phytopathogenic bacteria and fungi tested. Among the tested fungi Fusarium oxysporum f. sp. albedinis is the most sensitive to the actions of this Pseudomonas, where the inhibition rate reached 77.78%. The less sensitive one was Pythium ultimum with a rate of 55.56%. While for pathogenic bacteria only Salmonella enteridis was sensitive to the tested strain. Conclusion: Pseudomonas chlororaphis subsp aureofaciens showed appreciable antagonistic activity, in vitro, against special forms of Fusarium oxysporum and the tested phytopathogenic bacteria.
References (14)
- 1 Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities [DOI]
- 2 Role of a phenazine antibiotic from Pseudomonas fluorescens in biological control of Gaeumannomyces graminis var. tritici [DOI]
- 3 Occurrence, Biochemistry and Physiology of Phenazine Pigment Production [DOI]
- 4 Genetic analysis of the antifungal activity of a soilborne Pseudomonas aureofaciens strain [DOI]
- 5 Fusarium-wilt suppressive soils from the Châteaurenard region : review of a 10-year study [DOI]
- 6 Mechanisms of biocontrol of soil-borne plant pathogens by Rhizobacteria [DOI]
- 7 Multiple antibiotics produced by Pseudomonas fluorescens HV37a and their differential regulation by glucose [DOI]
- 8 Biocontrol of soil‐borne plant pathogens: Concepts and their application [DOI]
- 9 Quorum sensing systems of regulation, synthesis of phenazine antibiotics, and antifungal activity in rhizospheric bacterium pseudomonas chlororaphis 449 [DOI]
- 10 Influence of soil reaction on diversity and antifungal activity of fluorescent pseudomonads in crop rhizospheres [DOI]
- 11 Isolation, identification and antimicrobial activity of Pseudomonads isolated from the rhizosphere of potatoes growing in Algeria. [DOI]
- 12 Rapid quantitative analysis of phenazine-1-carboxylic acid and 2-hydroxyphenazine from fermentation culture of Pseudomonas chlororaphis GP72 by capillary zone electrophoresis [DOI]
- 13 Prevalence of fluorescent pseudomonads producing antifungal phloroglucinols and/or hydrogen cyanide in soils naturally suppressive or conducive to tobacco black root rot [DOI]
- 14 Bacteriocins Contributing in Rhizospheric Competition among Fluorescent Pseudomonads [DOI]
Cited by 2
M. Skowronek, E. Sajnaga, M. Pleszczyńska · International Journal of Molecular Sciences · 2020
D. Jošić, D. Cvikić, J. Zdravković · 2017
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