Changes of Carbonyl Stress Parameters in Rats with Diabetes and Rhabdomyolysis

Abstract

Aim: To investigate the changes of carbonyl stress parameters in rats with combined streptozotocin induced diabetes (STZD, hyperglycemia model) and glycerol stimulated rhabdomyolysis (RM, oxidative stress model). Methodology: RM was induced by glycerol injection and confirmed by changes of heme oxygenase activity, creatine kinase activity, total heme, creatinine, uric acid, urea and bilirubin. STZD was induced by streptozotocin injection and proved by increasing of glucose levels. Carbonyl stress development was determined by total aldehyde levels, TBARS, protein CO group levels, N(6)-Carboxymethyllysine and low-molecular-weight SH groups in blood plasma and liver tissue. Results: A significant increase of carbonyl stress parameters was recorded in RM and STZD rat groups compared with their relative control levels. In animals subjected to the combined induction of RM and STZD, the level of carbonyl stress parameters was lower than that recorded for the STZD and RM groups: total aldehyde levels were decreased in liver resulting in lower TBARS and protein CO group levels. Low-molecular-weight SH groups were increased compared with STZD. Conclusion: Changes of carbonyl stress parameters indicated the significant role of carbonyl stress in diabetes and in rhabdomyolysis. It was demonstrated that combined stimulation of RM and hyperglycemia led to decreasing of carbonyl stress parameters. It can be suggested that these changes in carbonyl stress parameters can be associated with additional initiation of antioxidant defense systems or some compensatory mechanisms.