Skip to content
Research Article Open access CC BY 4.0

Bactericidal and Bacteriostatic Effects of Four Different Parts of 2 Plants Moringa Extracts against the Bacteria Vibrio cholerae and Salmonella typhi, and some Phytochemical Properties

Claire Stéphane Metsopkeng, Geneviève Bricheux, Nola, Moïse, Florence Donnadieu, Paul Alain Nana, Isabelle Ripoche, Nehemie Donfagsiteli Tchinda, Gabriel A. Agbor, Pierre Chalard, Nana, Paul Alain, Télesphore Sime-Ngando, Tchinda, Nehemie Donfagsiteli, Moïse Nola, Bricheux, Geneviève

Journal of Advances in Microbiology · pp. 27–41 · Published 26 Aug 2023

10.9734/jamb/2023/v23i9749

Abstract

Background and Objectives: Typhoid fever and cholera are two major health challenges in many developing countries. The use of extracts of the plants Moringa for their treatment is often done without perfect knowledge of the antimicrobial properties of each part of plant. This study aims to evaluate the bactericidal and bacteriostatic effects of extracts of M. oleifera and M. stenopetala against the bacteria S. typhi and V. cholerae which cause typhoid fever and cholera respectively. Materials and Methods: Four different parts (leaves, seeds, stem, and roots) of each plant species were dried and then ground. The minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of the extracts of each part of the plants against the considered bacteria were determined by dilution method. Their phytochemical profiles were determined by chromatographic and mass spectral analyses. Results: It has been noted that MICs and MBCs varied from one part to another of the same Moringa species, and from one bacterium to another for the same extract. With M. oleifera, the lowest MBC (0.078 mg/mL) against S. typhi and V. cholerae came from seeds and roots extracts, respectively. With M. stenopetala, the lowest MBCs against S. typhi (0.156 mg/mL) and V. cholerae (0.312 mg/mL) were from seeds extracts. Extracts of different parts of M. oleifera have bactericidal effects against S. typhi, and bacteriostatic effects against V. cholerae. Extracts from all considered parts of M. stenopetala have bacteriostatic effects against both bacteria. Although some phytochemicals were found in both Moringa, others were specific to one species or to a given part. Conclusion:  In case of co-infection by both bacteria, M. oleifera roots extracts could be the most recommended in the treatment because of their lowest MIC and MBC. With M. stenopetala, roots and seeds extracts could be the most recommended because of their relatively lower MIC and MBC, respectively. The next studies will determine for each microorganism, the activity of each identified and purified chemical compound.  GRAPHICAL ABSTRACT

Plants M. oleifera and M. stenopetala plant part’s extract antimicrobial activities S. typhi and V. Cholerae MICs and MBCs phytochemicals

References (46)

  1. 1 Determination of minimum inhibitory concentrations [DOI]
  2. 2 Molecular mechanisms of antibiotic resistance [DOI]
  3. 3 Antifungal Agents: Mode of Action, Mechanisms of Resistance, and Correlation of These Mechanisms with Bacterial Resistance [DOI]
  4. 4 The effect of developmental and environmental factors on secondary metabolites in medicinal plants [DOI]
  5. 5 Ethanolic Extract of Propolis (EEP) Enhances the Apoptosis- Inducing Potential of TRAIL in Cancer Cells [DOI]
  6. 6 Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases [DOI]
  7. 7 Antibacterial Properties of Polyphenols: Characterization and QSAR (Quantitative Structure–Activity Relationship) Models [DOI]
  8. 8 Organic acids as antimicrobials to control Salmonella in meat and poultry products [DOI]
  9. 9 Pharmacokinetics and Pharmacodynamics of Antibacterial Agents [DOI]
  10. 10 Antibacterial fatty acids: An update of possible mechanisms of action and implications in the development of the next-generation of antibacterial agents [DOI]
  11. 11 Antibacterial activities of the extracts, fractions and isolated compounds from Canarium patentinervium Miq. against bacterial clinical isolates [DOI]
  12. 12 Structural origins of gentamicin antibiotic action [DOI]
  13. 13 Moringa oleifera: An Updated Comprehensive Review of Its Pharmacological Activities, Ethnomedicinal, Phytopharmaceutical Formulation, Clinical, Phytochemical, and Toxicological Aspects [DOI]
  14. 14 Proximate and ultimate causes of the bactericidal action of antibiotics [DOI]
  15. 15 Moringa oleifera is a Prominent Source of Nutrients with Potential Health Benefits [DOI]
  16. 16 Prescott's Microbiology
  17. 17 Antimicrobial flavonoids as a potential substitute for overcoming antimicrobial resistance [DOI]
  18. 18 Antibiotic resistance in Vibrio cholerae: Understanding the ecology of resistance genes and mechanisms [DOI]
  19. 19 Antioxidant and Anti-Inflammatory Activities of the Crude Extracts of Moringa oleifera from Kenya and Their Correlations with Flavonoids [DOI]
  20. 20 Selective Antimicrobial Activities and Action Mechanism of Micelles Self-Assembled by Cationic Oligomeric Surfactants [DOI]
  21. 21 Antibacterial properties of quercetin [DOI]
  22. 22 Antimicrobial activities of flavonoid glycosides from Graptophyllum grandulosum and their mechanism of antibacterial action [DOI]
  23. 23 Genomic plasticity associated with antimicrobial resistance in Vibrio cholerae [DOI]
  24. 24 Comparative Analysis of Chemical Constituents of Moringa oleifera Leaves from China and India by Ultra-Performance Liquid Chromatography Coupled with Quadrupole-Time-Of-Flight Mass Spectrometry [DOI]
  25. 25 Discovery of 4,5,6,7-Tetrahydrobenzo[1,2- d ]thiazoles as Novel DNA Gyrase Inhibitors Targeting the ATP-Binding Site [DOI]
  26. 26 Weak acids as an alternative anti-microbial therapy [DOI]
  27. 27 Intact Glucosinolate Analysis in Plant Extracts by Programmed Cone Voltage Electrospray LC/MS: Performance and Comparison with LC/MS/MS Methods [DOI]
  28. 28 Antibacterial Activity of Glucomoringin Bioactivated with Myrosinase against Two Important Pathogens Affecting the Health of Long-Term Patients in Hospitals [DOI]
  29. 29 Mechanisms of Antibiotic resistance in Salmonella typhi
  30. 30 Cytotoxic and antibacterial activities of iridoids and sesquiterpenoids from Valeriana jatamansi [DOI]
  31. 31 Antimicrobial activity of different sodium and potassium salts of carboxylic acid against some common foodborne pathogens and spoilage-associated bacteria [DOI]
  32. 32 Isolation and identification of two new compounds from the seeds of Moringa oleifera and their antiviral and anti-inflammatory activities [DOI]
  33. 33 Optimized methods for detecting Salmonella Typhi in the environment using validated field sampling, culture and confirmatory molecular approaches [DOI]
  34. 34 Potential therapeutic uses ofMoringa stenopetala: a scoping review [DOI]
  35. 35 Characterisation of Moringa stenopetala seed oil variety “Marigat” from island Kokwa [DOI]
  36. 36 Basal and Spasmolytic Effects of a Hydroethanolic Leaf Extract of Melissa officinalis L. on Intestinal Motility: An Ex Vivo Study [DOI]
  37. 37 Xanthine Oxidase Inhibitors from Filipendula ulmaria (L.) Maxim. and Their Efficient Detections by HPTLC and HPLC Analyses [DOI]
  38. 38 Purification, Fermentation Optimization, and Antibacterial Activity of Pyrrole-2-carboxylic Acid Produced by an Endophytic Bacterium, Bacillus cereus ZBE, Isolated from Zanthoxylum bungeanum [DOI]
  39. 39 Quercetin-3-Rutinoside Blocks the Disassembly of Cholera Toxin by Protein Disulfide Isomerase [DOI]
  40. 40 Antimicrobial effect of Moringa oleifera seed powder against Vibrio cholerae isolated from the rearing water of shrimp ( Penaeus vannamei ) postlarvae [DOI]
  41. 41 ANALYSIS OF PHENOLIC ACIDS AND ANTIBACTERIAL ACTIVITY OF EXTRACTS OBTAINED FROM THE FLOWERING HERBS OF CARD UUS ACANTHOIDES L.
  42. 42 Common plants used in the treatment of typhoid fever, their active components and toxicity related issues: A review [DOI]
  43. 43 Medicinal plants for the treatment of malaria and typhoid diseases [DOI]
  44. 44 Risques microbiologiques alimentaires
  45. 45 MINIMUM INHIBITION AND BACTERICIDAL CONCENTRATIONS OF THE PLANT MORINGA OLEIFERA EXTRACTS AGAINST THE BACTERIA ESCHERICHIA COLI AND STAPHYLOCOCCUS AUREUS [DOI]
  46. 46 Moringa oleifera (horse radish tree) [DOI]

Cited by 4

Investigating Dysbiosis‐Driven Colonization by Staphylococcus aureus and the Mitigative Potential of Medicinal Plants in Diabetes‐Linked Inflammation

Kehinde Abel Ajadi, Oluwapelumi Oyindamola Ogunleye, Adeogo Oreoluwa Jibodu · Advanced Gut & Microbiome Research · 2026

Supplementation of Moringa oleifera leaves improves the safety and quality of fermented shrimp paste

R Surya, E H Megumi, D Nugroho · IOP Conference Series: Earth and Environmental Science · 2024

Showing 2 of 4 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

7

Page views

0

PDF downloads

0

Outbound clicks

4

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.