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Research Article Open access CC BY 4.0

Assessment of the Methanogenic Potential of Biogas Produced by Anaerobic Co-digestion of Three Mixing Ratios of Seaweed Waste (Sargassum spp.) and Pig Manure

Emmanuel Gozoua, Kouamé Innocent Kouadio, Tano Ladji Acka, Drissa Sylla, Youan Charles Tra Bi, Boko Aka

Biotechnology Journal International · pp. 97–104 · Published 2 Nov 2026

10.9734/bji/2026/v30i6925

Abstract

Objective: The aim of this study was to evaluate the methanogenic potential of biogas produced by anaerobic co-digestion using three different mixing ratios of seaweed waste (Sargassum spp.) and pig manure. Methodology: Biogas production was carried out using an experimental water-displacement system. Three experimental set-ups were prepared using three mixing ratios of the substrate and co-substrate. The first bioreactor was fed with a mixture comprising 30 % of seaweed waste and 70 % of pig manure (30:70). The second set-up was fed with 50 % of seaweed waste and 50 % of pig manure (50:50), and the third comprised 70 % of seaweed waste and 30 % of pig manure (70:30). The fermentation process lasted 32 days and was carried out in batch mode. The composition of the biogas produced from each mixture was analysed, and flammability tests were performed. Results: The results showed that the cumulative volume of biogas for the 30:70, 50:50 and 70:30 ratios was 16.5 L, 17.8 L and 5.4 L, respectively. The biogas produced at the 30:70 ratio exhibited the highest methane content, at 60 %, while those produced at the 50:50 and 70:30 ratios had methane contents of 45 % and 36 %, respectively. In addition, the biogas flammability test showed a blue flame for the biogas produced at the 30:70 ratio, whereas no blue flame was observed for the biogas produced from the other two mixtures. Conclusion: The biogas produced from the mixture containing 30 % seaweed waste and 70 % pig manure therefore showed the highest observed methane fraction. This study suggests a possible route for recovering energy from seaweed strandings while addressing coastal waste management.

Seaweed; pig manure co-digestion biogas methanogenic potential water-displacement system flammability

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