Skip to content
Research Article Open access CC BY 4.0

Application of Trichoderma viride and Bacillus subtilis Leads to Changes in Antioxidant Enzymes, Proline, and Lipid Peroxidation under Salinity Stress in Chickpea (Cicer arietinum L.)

. Kavita, Nagaram Sowmya

International Journal of Plant & Soil Science · pp. 187–197 · Published 3 Nov 2021

10.9734/ijpss/2021/v33i2230695

Abstract

The study was carried out to evaluate the influence of application of Trichoderma viride and Bacillus subtilis on antioxidant enzymes, proline and lipid peroxidation to decrease the impact of salinity stress on chickpea (Cicer arietinum L.), a salinity sensitive crop. A pot experiment was conducted with contrasting set of genotypes (tolerant vs. sensitive) under salinity stress compared to control soil conditions in completely randomized design with three replications. Microbial inoculation was done through seed priming and application to soil at 20 days after sowing (DAS). Content of antioxidant enzymes, proline, and lipid peroxidation were assessed in leaves at flowering stage. Results showed that antioxidant enzymes viz., catalase, peroxidase, and superoxide dismutase were significantly increased under salinity stress compared to control condition and they were further increased with application of microbes either as seed priming alone or in combination with soil application at 20 DAS in both the genotypes under saline as well as control conditions. The content of lipid peroxidation increased significantly under salinity stress, and it was stronger pronounced in sensitive genotype while the lipid peroxidation content was decreased by application of microbes. Proline content increased under salinity stress, and it was further enhanced by the microbial inoculation. The study thus conclusively proved that Bacillus subtilis and Trichoderma viride positively increased content of antioxidant enzymes, proline, and lipid peroxidation in leaves of chickpea grown under salinity stress conditions. The best microbe species was Trichoderma viride as seed priming plus soil application. This can be an important additional approach to decrease the impact of salinity stress on chickpea crop.

Chickpea salinity stress antioxidant Bacillus subtilis Trichoderma viride

References (19)

  1. 1 Rapid determination of free proline for water-stress studies [DOI]
  2. 2 Leaf Senescence: Correlated with Increased Levels of Membrane Permeability and Lipid Peroxidation, and Decreased Levels of Superoxide Dismutase and Catalase [DOI]
  3. 3 Learning from television in young children [DOI]
  4. 4 Induced Systemic Resistance and Plant Responses to Fungal Biocontrol Agents [DOI]
  5. 5 Bacillus: A Biological Tool for Crop Improvement through Bio-Molecular Changes in Adverse Environments [DOI]
  6. 6 Insights into the significance of antioxidative defense under salt stress [DOI]
  7. 7 Effect of arbuscular mycorrhizal fungi on growth, mineral nutrition, antioxidant enzymes activity and fruit yield of tomato grown under salinity stress [DOI]
  8. 8 Microbial amelioration of crop salinity stress [DOI]
  9. 9 Role of Trichoderma harzianum in mitigating NaCl stress in Indian mustard (Brassica juncea L) through antioxidative defense system [DOI]
  10. 10 Differential response of salt stress on Brassica juncea: Photosynthetic performance, pigment, proline, D1 and antioxidant enzymes [DOI]
  11. 11 Salt sensitivity in chickpea [DOI]
  12. 12 Measuring Soil Salinity [DOI]
  13. 13 Physiological and biochemical parameters for evaluation and clustering of rice cultivars differing in salt tolerance at seedling stage [DOI]
  14. 14 Trichoderma—not just for biocontrol anymore [DOI]
  15. 15 Salt-tolerant Bacillus species as a promising strategy to mitigate the salinity stress in wheat (Triticum turgidum subsp. durum) [DOI]
  16. 16 Effect of Fe Deficiency on Antioxidant System in Leaves of Three Flax Cultivars [DOI]
  17. 17 Mitigation of drought in rice by a phyllosphere bacterium Bacillus altitudinis FD48 [DOI]
  18. 18 Bacillus subtilis improves maize tolerance to salinity [DOI]
  19. 19 Trichoderma-Induced Enhancement of Soybean Seedling Performance in Response to Salt Stress

Cited by 1

1 citation reported by external sources — individual citing-article records aren't available to list yet.

Related research

Article metrics

Real usage data collected on this platform.

32

Page views

0

PDF downloads

13

Outbound clicks

1

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.