Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts
Ermina Cilović Kozarević, Esmeralda Dautović, Dalila Halilčević, Adaleta Softić, Nahida Srabović, Broza Šarić-Kundalić, Nina Delić, Lamija Kolarević, Lejla Mekić, Merima Ibišević, Emir Horozić, Jelena Arsenijević, Zoran Maksimović
International Research Journal of Pure and Applied Chemistry · pp. 23–32 · Published 30 Sep 2022
10.9734/irjpac/2022/v23i5788Abstract
Aims: The aims of the study were to analyse the polyphenols of Pulicaria dysenterica (L.) Bernh. methanolic extracts from aerial and underground parts, assessment of antioxidant activity and to evaluate their cytotoxicity on HeLa cells of cervical cancer. Methodology: The total phenolic content (TPC) of extracts was determined by the Folin-Ciocalteu spectrophotometric method. The qualitative and quantitative analysis of individual polyphenolic compounds were performed by the reverse phase HPLC method. The antioxidant capacity was evaluated by both, 2,2-diphenyl1-picrylhydrazyl radical and FRAP assay, while cytotoxicity of the extracts was assessed by MTT assay. Results: TPC of the samples were 127.62±2.22 and 244.12±8.84 mg gallic acid equivalent/g extract. In the extracts chlorogenic acid in amount of 10.06±0.96 and 11.32±0.28 mg/g, flavonoid rutin in amount of 5.68±0.13 mg/g and three caffeic acid derivatives were recorded. Extract from underground parts achieved better antioxidant activity with IC50 value 55.36±0.75 µg/mL and FRAP value 2411.12±37.22 µmol Fe2+g-1 compared to the one from aerial parts. Extract from aerial parts achieved better cytotoxic activity with 50% inhibition of viability (IC50) at concentration of 0.389±0.07 mg/mL, against HeLa cells, compared to the extract from underground parts. Conclusion: Analyzed Pulicaria dysenterica extracts contained phenolic acids and flavonoids. The extracts showed good antioxidant activity and cytotoxic properties against HeLa cells in vitro.
References (31)
- 1 Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method [DOI]
- 2 Rutin: A Flavonoid as an Effective Sensitizer for Anticancer Therapy; Insights into Multifaceted Mechanisms and Applicability for Combination Therapy [DOI]
- 3 Rutin inhibits proliferation, attenuates superoxide production and decreases adhesion and migration of human cancerous cells [DOI]
- 4 CANCER CELL CULTURE—METHODS AND PROTOCOLS [DOI]
- 5 Chlorogenic acid induces reactive oxygen species generation and inhibits the viability of human colon cancer cells [DOI]
- 6 Anticancer Activity of Rutin and Its Combination with Ionic Liquids on Renal Cells [DOI]
- 7 A novel rutin-fucoidan complex based phytotherapy for cervical cancer through achieving enhanced bioavailability and cancer cell apoptosis [DOI]
- 8 Chlorogenic acid complex (CGA7), standardized extract from green coffee beans exerts anticancer effects against cultured human colon cancer HCT-116 cells [DOI]
- 9 Chlorogenic acid inhibits Bcr-Abl tyrosine kinase and triggers p38 mitogen-activated protein kinase–dependent apoptosis in chronic myelogenous leukemic cells [DOI]
- 10 Antioxidant action and cytotoxicity on HeLa and NIH-3T3 cells of new quercetin derivatives [DOI]
- 11 Antioxidant potential of Salvia officinalis L. residues as affected by the harvesting time [DOI]
- 12 Guaianolide sesquiterpenes from Pulicaria crispa (Forssk.) Oliv. [DOI]
- 13 Synergetic Impact of Combined 5-Fluorouracil and Rutin on Apoptosis in PC3 Cancer Cells through the Modulation of P53 Gene Expression [DOI]
- 14 Caffeic acid and chlorogenic acid cytotoxicity, genotoxicity and impact on global DNA methylation in human leukemic cell lines [DOI]
- 15 Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line [DOI]
- 16 Evaluation of the Cytotoxic Effect of Rutin Prenanoemulsion in Lung and Colon Cancer Cell Lines [DOI]
- 17 Assessment of anticancer activity of Pulicaria undulata on hepatocellular carcinoma HepG2 cell line [DOI]
- 18 Rutin Mediated Apoptotic Cell Death in Caski Cervical Cancer Cells via Notch-1 and Hes-1 Downregulation [DOI]
- 19 CYTOTOXIC AND ANTIOXIDANT ACTIVITIES OF SECONDARY METABOLITES FROM PULICARIA UNDULATA [DOI]
- 20 Innovative perspectives on Pulicaria dysenterica extracts: phyto‐pharmaceutical properties, chemical characterization and multivariate analysis [DOI]
- 21 Interaction of curcumin on cisplatin cytotoxicity in HeLa and HepG2 carcinoma cells [DOI]
- 22 Evaluation of anti-proliferative and cytotoxic effects of chlorogenic acid on breast cancer cell lines by real-time, label-free and high-throughput screening [DOI]
- 23 Antibacterial activity of Pulicaria dysenterica extracts [DOI]
- 24 Essential Oil Composition ofPulicaria dysenterica(L.) Bernh from Iran [DOI]
- 25 In vitro anti-parasitic activities of Pulicaria dysenterica and Lycopus europaeus methanolic extracts against Trichomonas gallinae [DOI]
- 26 Phytochemicals Analysis and Some Bioactive Properties of Erica manipuliflora Salisb. (EMS); Antibacterial, Antiradical and Anti-lipid Peroxidation. [DOI]
- 27 Geographical variation in the surface flavonoids of Pulicaria dysenterica [DOI]
- 28 Chemical Composition ofPulicaria dysenterica(L.) Bernh. from Greece [DOI]
- 29 Chemical Constituents of Tibetan Herbal Medicine Pulicaria insignis and Their in vitro Cytotoxic Activities [DOI]
- 30 Screening of Some Apiaceae and Asteraceae Plants for Their Cytotoxic Potential [DOI]
- 31 Antioxidative and antimicrobial activity of Reynoutria japonica extracts [DOI]
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