Anticariogenic Activity of Selenium Nanoparticles with Pterocarpus santa

Abstract

Introduction: Nanoparticles (NPs) are used to minimise toxicity, increase bioactivity, improve targeting, and monitor the release profile of the encapsulated moiety in a variety of ways. Inorganic NPs such as Ag, Au, Ce, Fe, Se, Ti, and Zn hold a special position among NPs due to its versatile bioactivities in nanoforms.Selenium (Se) is an essential trace mineral.SeNPs have been studied in the treatment of oxidative stress and inflammation-related diseases such as arthritis, cancer, diabetes, and nephropathy.Medicinal plants have long been a valuable source of natural active constituents that have been used in products for preserving human health. Pterocarpus santalinus (Fabaceae) has been used as a folk medicine and has been shown to have anti-inflammatory, anti- ulcer, and anti-cancer properties. The aim of the present work is to evaluate synergistic Anticariogenic properties of Pterocarpus santalinus (red sandalwood). Materials and Methods: Anticariogenic activity of respective nanoparticles against the strain staphylococcus aureus, Candida albicans, and Enterococcus faecalis and s.mutans . MHA agar was utilized for this activity to determine the zone of inhibition. Muller Hinton agar was prepared and sterilized for 45 minutes at 120lbs. Media poured into the sterilised plates and let them stabilize for solidification. The wells were cut using the well cutter and the test organisms were swabbed.The nanoparticles with different concentrations were loaded and the plates were incubated for 24 hours at 37°C. After the incubation time the zone of inhibition was measured. Results: Agar well diffusion demonstrated that Pterocarpus santa significantly inhibited the growth of S.Aureus, S.mutans, E. faecalis and C.albicans . The increase in concentration increases the zone of inhibition.Data were implied as mean ± SEM with the level of statistical significance at p<0.05. Conclusion: Selenium nanoparticles were synthesised from Pterocarpus santa and they are characterised .Their special study showed the potent anticariogenic activity against pathogens.