Green Synthesis of Zinc Oxide NPS Using Boerhavia diffusa and Its Anticariogenic Activity

Abstract

Introduction: Boerhavia diffusa (BD) is a plant of rasayana category as per ayurvedic claims. It is reported to possess anticariogenic activity, disease prevention, and life strengthening activities which hold enormous influence in disease burden and affordability/availability of healthcare in the world. Materials and Methods: 1g of Boerhavia diffusa is mixed 100 ml of distilled water and  2.5(0.514)g of (20 micromolar boerhavia diffusa) was dissolved in 60ml of distilled water  to that 40 ml of filtered plant extract was added and  was kept in an orbital shaker for approximately 72 hours. The formation of nanoparticles were confirmed both visually and by UV visible spectrophotometer. The nanoparticles were then centrifuged with the aid of a lark refrigerator centrifuge for 10 minutes at 8000 rpm. Results and Discussion: Anticariogenic activity of respective nanoparticles against the strain staphylococcus aureus, Candida albicans, Enterococcus faecalis and Streptococcus mutans was utilized for this activity to determine the zone of inhibition. Muller hinton agar  was prepared and sterilized for 45 min at 120Ibs. The media was poured into the sterilized playesa d was let to stabilize for solidification. The wells were cut using the well cutter and the test organism was swabbed. Conclusion: Anticariogenic activity of respective nanoparticles against the strain staphylococcus aureus, Candida albicans, Enterococcus faecalis and Streptococcus mutans was utilized for this activity to determine the zone of inhibition. Muller hinton agar  was prepared and sterilized for 45 min at 120Ibs. The media poured into the sterilized plates was let to stabilize for solidification. The wells were cut using the well cutter and the test organism was swabbed.