Anti-microbial Properties of Valeriana officinalis, Satureja bachtiarica and Thymus daenensis Methanolic Extracts against Helicobacter pylori

Abstract

Aims: Helicobacter pylori infection spreads widely in the world and has several complications including gastric cancer. The aim of this work is to investigate the antimicrobial properties of Valeriana officinalis L., Satureja bachtiarica and Thymus daenensis extracts against H. pylori. Methods: The disk diffusion test was used in this experiment. Methanolic extract was isolated from Valeriana officinalis L., Satureja bachtiarica and Thymus daenensis. Ten clinical isolates of H. pylori were used in this experiment, which was obtained at Alzahra hospital, Tehran, Iran from individual patients with gastrointestinal disorders. H. pylori strains were identified using the checking of bacterial growth (0.5 – 1 mm), Gram staining, urease test, catalase test and the drug resistance standard was performed on them. To determine the MIC of the extracts, disk diffusion (Kirby-Bauer) test in agar was used. Results: The clinical strains showed the highest susceptibility to tetracycline (10%). The inhibition zone diameter was the highest at 10% concentration of methanolic extracts of Valeriana officinalis L., Satureja bachtiarica and Thymus daenensis and had the highest growth inhibitory effect at higher concentrations. Conclusion: The methanolic extracts of Valeriana officinalis L., Satureja bachtiarica and Thymus daenensis herbs showed high anti-H. pylori effects in high concentrations and among them, Satureja bachtiarica showed the highest antibacterial effect.